大剂量氨溴索对海水浸泡致急性肺损伤犬体内SP-A、SP-B含量的影响

Effect of high-dose ambroxol on surfactant protein A and B of dogs with acute lung injury following sea water immersion

目的:探讨大剂量氨溴索对胸部开放伤后海水浸泡致急性肺损伤(ALI)犬体内肺表面活性蛋白A和B(SP-A、SP-B)含量的影响。方法:胸腔注入海水制备犬ALI模型,随机分为对照组(CG)及氨溴索治疗组(AG),AG犬在海水浸泡10min后静脉注射12mg/kg的氨溴索,CG组以等量生理盐水代替,观察伤后不同时间点血清及肺组织中SP-A、SP-B含量的变化。结果:致伤后6h后氨溴索治疗组及对照组血清中SP-A、SP-B含量均较实验前明显增高(P<0.01),但两组间无统计学差异(P>0.05);而氨溴索治疗组支气管肺泡灌洗液(BALF)中SP-A、SP-B的含量明显高于对照组(P<0.01)。结论:氨溴索能诱导胸部开放伤后海水浸泡致ALI犬肺内SP-A、SP-B的表达。

Objective： To investigate the effects of high-dose ambroxol on the alteration of surfactant A and B of dogs with acute lung injury following open chest trauma and sea water immersion. Methods： Animal model of ALI was established by seawater immersed thoracic cavity after open chest trauma. These dogs were assigned randomly into the control group（CG） and the ambroxol treatment group（AG）. Dogs in AG group had intravenous injection with 12 mg/kg body weight of ambroxol 30 min after open chest trauma and sea water immersion; dogs in CG group were treated with equal normal saline. Arterial blood gas, extravascular lung water index and Evan＇s blue leakage index were determined in 2 groups. Expressions of SP-A and SP-B in lung and blood serum on different times were also measured. Results： At 6 h after treatment, the PaO2 and PaO2/ FiO2 in DG group was significantly higher than control group （P〈0.05）, the EVL-WI and ELI in DG group was lower than control group（P〈0.05）, the SP-A and SP-B in DG group in bronchoalveolar lavage fluid was higher than CG guoup and SP-A and SP-B in blood serum lower than CG group（P〈0.01）. Conclusion： Dexamethasone can relieve hypoxia and pulmonary edema after open chest trauma and sea water immersion in dogs and increase the concentrations of SP-A and SP-B in lungs.