摘要
目的研究多聚异戊二烯基苯甲酮（garcinol）对宫颈癌Hela细胞株放射敏感性的影响。方法不同浓度garcinol作用于人宫颈癌Hela细胞12、24、48h，CCK-8法检测garcinol对Hela细胞增殖活性的影响，并计算出24h时的20％和50％的药物抑制浓度（IC20和IC50）值；克隆形成实验检测20Ixmol／Lgarcinol对Hela细胞放射敏感性的影响；反转录PCR法和Westernblot法检测Hela细胞内环氧化酶-2（cyclooxygenase-2，COX-2）在mRNA水平和蛋白表达水平的变化。结果garcinol抑制宫颈癌Hela细胞的生长，并且有明显的时间-剂量依赖性；通过单击多靶模型计算放射生物学参数，经20μmol／Lgarcinol处理24h的Hela细胞，与单纯照射组相比，放射敏感性增加（t=6．69，P〈0．05），放射增敏比（SER）为1．26；经20μmol／Lgarcinol处理24h的Hela细胞，COX-2的蛋白表达水平下降（P〈0．05），而COX-2mRNA水平无明显变化（P〉0．05）。结论garcinol可以增加宫颈癌Hela细胞的放射敏感性，这可能与其降低Hela细胞中COX-2的蛋白表达水平有关。
Objective To investigate the effects of polyisoprenylated benzophenone （garcino） on the radiosensitivity of human cervical cancer Hela cells. Methods Hela cells were exposed to different concentrations of garcinol for 12, 24 and 48 hours. The proliferative rate of the cells was detected by CCK - 8 assay, while the values of IC20 and IC50 were calculated after 24 hours of incubation. Clonogenie survival assay was adopted to evaluate the effects of 20 p, mol/L garcinol on the radiosensitivity of Hela cells. The levels of COX - 2 mRNA and protein were measured by RT - PCR and Western blotting, respectively. Results Garcinol could inhibit the growth of Hela cells in time - and dose - dependent ways. Incubation with 20μmol/L garcinol for 24 house stimulated the radiosensitivity of the cells （ t = 6.69, P 〈 0.05 ） , with a sensitizing enhancement ratio （SER） of 1.26. The level of COX - 2 protein in Hela cells was decreased after treatment with garcinol （20μmol/L） for 24 hours （P 〈 0.05）, while no changes were observed as to the amount of Cox - 2 mRNA （P 〉 0.05 ）. Conclusion Garcinol can enhance the radiosensitivity of Hela cells, which may be related with the down - regulated level of COX - 2 protein.
出处
《徐州医学院学报》
CAS
2014年第7期430-433,共4页
Acta Academiae Medicinae Xuzhou
基金
徐州市科技局科研基金（XM09B103）
