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四川地区北京型结核分枝杆菌的MIRU-VNTR基因分型 被引量：6

Genotyping of Beijing family of mycobacterium tuberculosis isolates from sichuan with MIRU-VNTR
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摘要 用寡核苷酸多态性基因分型(Spoligotyping)对2008年1月至2010年10月间采集的211株结核分枝杆菌临床分离株进行基因分型,然后对北京型结核分枝杆菌VNTR位点的多态性进行检测.选用的12个VNTR位点存在较高的基因多态性,该12位点组合分辨能力(HGI=0.99985)高于标准的VNTR-12位点组合(HGI=0.99546),其中多态性较高的7位点组合HGI指数为0.99977.因此,7位点组合可以作为四川地区北京家族分枝杆菌的一线分型方法. Clinical M. tuberculosis isolates from Jan 2008 to Oct 2010 were genotyped by Spoligotyping, and Beijing family was further analyzed for the polymorphism of the twelve tandem repeat loci. Analysis based on MIRU-VNTR for 162 Beiiing isolates suggested that the selected 12 loci show a higher HGI value compared with the original 12 loci method. Using 7-locus VNTR of high polymorphism, the HGI value （0.9998） was obtained. It is suggested that the method of 7-locus can be used as the first-line mo- lecular typing tool for Beijing family isolates of M. tuberculosis.
作者 冯钦 景誉庆 黄正辉 段菁岳 郑超 孙宏虎 钟晶 孙群
机构地区 四川大学生命科学学院生物资源与生态环境教育部重点实验室 成都市结核病防治院
出处 《四川大学学报（自然科学版）》 CAS CSCD 北大核心 2014年第4期851-856,共6页 Journal of Sichuan University(Natural Science Edition)
基金 国家自然科学基金(J1103518)
关键词 结核分枝杆菌 北京家族 寡核苷酸 数目可变串联重复序列 四川地区 Mycobacterium tuberculosis Beijing family Oligonucleotides VNTR Sichuan area
分类号 R378.911 [医药卫生—病原生物学]
  • 相关文献

参考文献14

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二级参考文献25

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共引文献10

同被引文献63

  • 1石荔,杨敏,Christine Pourcel,董海燕,尼玛彭多,张嫒媛,朗珍,吕冰,蒋毅,魏淑贞,范昕建,万康林.MLVA和Spoligotyping用于西藏地区216株结核分枝杆菌临床分离株的基因分型研究[J].中华微生物学和免疫学杂志,2007,27(8):711-718. 被引量：19
  • 2许卫国,虞浩,杨丹丹,吕华坤,周扬.数目可变串联重复序列用于江苏省168株结核分枝杆菌菌株的基因分型研究[J].南京医科大学学报（自然科学版）,2007,27(12):1509-1512. 被引量：8
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引证文献6

二级引证文献21

四川大学学报（自然科学版）
2014年 第4期

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