胃腺癌血管活性肠肽受体mRNA表达的研究

Vasoactive intestinal peptide receptor mRNA expression in gastric adenocarcinoma

目的研究血管活性肠肽受体mRNA在胃腺癌组织和AGS人胃腺癌细胞中的表达情况,探讨其在胃腺癌发病过程中的作用。方法采用RT-PCR方法,检测24例胃腺癌组织、24例正常胃腺组织及不同传代AGS人胃腺癌细胞中VIPR mRNA的表达。结果胃腺癌患者组VIPR1 mRNA表达率为45.00%(9/24),低于正常对照组的83.33%(20/24)(P<0.05);VIPR2 mRNA表达率在胃腺癌患者组和正常对照组分别为50.00%(10/24)和66.67%(16/24),差异无统计学意义(P>0.05)。与正常胃窦黏膜比较,VIPR1 mRNA的表达量下降(P<0.05),但VIPR2 mRNA的表达量和正常胃窦黏膜差异无统计学意义(P>0.05)。不同传代AGS人胃腺癌细胞中均有VIPR mRNA的表达,但传代细胞之间差异无统计学意义(P>0.05)。结论VIPR mRNA的低表达可能与胃腺癌的发生发展有关,检测VIPR mRNA表达对胃腺癌的诊断具有一定的指导意义。

Objective To investigate the expression of vasoactive intestinal peptide receptor （ VIPR） mRNA in gastric adenocarcinoma and AGS cells , and understand the effect of VIPR on gastric adenocarcinoma and possible mechanism.Methods Gastric tissues of 24 patients with gastric adenocarcinoma and 24 healthy controls were collected, and the expression of VIPR mRNA was determined by real time PCR .The VIPR mRNA expression was also detected in gastric adenocarcinoma cell line ACG by the same way .Results The expression rate of VIPR1 mRNA was 45.00%（9/24）in gastric adenocarcinoma, which was significantly lower than controls 83.33%（20/24）（P〈0.05 ） , but that of VIPR2 mRNA didn′t changed significantly （ 50.00% vs 66.67% ） （ P〉0.05 ） .The levels of VIPR1 mRNA in gastric adenocarcinoma was lower than that in controls （ P〈0.05 ） , while there was no different in the levels of VIPR2 mRNA（P〉0.05）.All of the AGS cells were positive both in VIPR1 and VIPR2 mRNA expression.Conclusion The lower level of VIPR mRNA expression may be an integral part in the pathogenesis of gastric adenocarcinoma , and VIPR mRNA detection may be contribute to the diagnosis of gastric carcinoma .