囊胚期胚胎活检及玻璃化冻存的研究

Impact of blastocyst biopsy on the embryos development potential and survival rate after vitrification

目的探讨不同质量囊胚活检后的继续发育潜力和玻璃化冻融后的复苏能力。方法选择体外受精-胚胎移植周期患者的剩余胚胎,根据授精后第5/6天囊胚质量等级分为高质量囊胚组和低质量囊胚组,两组囊胚根据活检与否分别分为活检组和对照组。其中活检组囊胚采用激光切割结合抽吸法活检滋养层细胞,而后将活检成功囊胚进行玻璃化冷冻;对照组囊胚不活检,直接进行玻璃化冷冻。比较不同分组囊胚的活检结局及玻璃化冻融效果。结果低质量囊胚活检成功率低于高质量囊胚,但差异无统计学意义(P=0.183),低质量囊胚活检后玻璃化冻融复苏率显著低于高质量囊胚(P=0.001),但低质量囊胚和高质量囊胚活检组的玻璃化冻融复苏率与对照组相比差异均无统计学意义(P=0.597,P=0.823)。结论囊胚期活检不影响胚胎继续发育能力及玻璃化冻融复苏效果。

Objective To investigate the impact of blastocyst biopsy on the development potential and survival rate after vitrification of different quality blastocysts .Methods The surplus embryos in IVF-ET cycles were allocated to good or poor quality blastocysts group by the embryo grade in day 5/6, and the same quality blastocysts were allocated to biopsy and control group .For the blastocysts from biopsy group trophectoderm removal was performed and then the survival blastocysts were vitrificated , but for control group vitrification was performed without biopsy.The survival rate after blastocyst biopsy and warming were compared between different groups .Results For the poor quality blastocysts group the survival rate after blastocyst biopsy was lower than the good quality blastocysts group, but the difference was not significant （ P=0.183 ） , and the survival rate after warming was significantly lower than the good quality blastocysts group （ P=0.001 ） .But the survival rate after warming was similar between the biopsy group and control group（P=0.597,P=0.823）.Conclusion Blastocyst biopsy did not impair the development potential and survival efficient after vitrification .