基于核磁共振的甲状腺乳头状癌患者血浆的代谢组学研究

A plasma metabolomics study of papillary thyroid cancer with NMR

目的通过核磁共振代谢组学方法进行甲状腺乳头状癌患者与健康对照者血浆代谢组研究,并探讨甲状腺乳头状癌组代谢组学上的变化及意义。方法收集健康对照组(n=29)和甲状腺乳头状癌组(n=31)血浆样品,进行核磁共振氢谱(1 H-NMR)检测。通过最小二乘判别分析(OPLS-DA)方法得到两组血浆的差异性代谢物,并探讨相关生化过程的改变。结果两组血浆代谢物有明显的差异,与健康对照组相比,甲状腺乳头状癌组血浆中的极低密度脂蛋白、异亮氨酸、亮氨酸、缬氨酸、低密度脂蛋白、乳酸、丙氨酸、糖蛋白、谷氨酰胺、肉碱、α-酮戊二酸、肌酸、甘氨酸、不饱和脂类、酪氨酸、甲基组氨酸、苯丙氨酸的含量降低,丙酮酸、柠檬酸、肌酸酐、胆碱、鲨肌醇、β-葡萄糖、肌醇、α-葡萄糖和甲酸的含量增高,差异有统计学意义(P<0.05)。结论根据血浆代谢物的差异可以分辨健康对照组与甲状腺乳头状癌组血浆中的差异性代谢物。这种基于核磁共振氢谱技术的代谢组学方法可为甲状腺乳头状癌的诊断提供可靠代谢依据。

Objective Nuclear magnetic resonance （NMR）was used to study of papillary thyroid carcinoma group′s blood plasma and health group′s blood plasma metabolomics and their significance.Methods Blood plasma of healthy people group （n=29）and patients group with papillary thyroid carcinoma （n=3 1 ）were collected and were analyzed by the method of ^1 H-NMR .Different metabolites of two sets of plasma were obtained by using orthogonal proj ections to latent structures discriminant analysis （OPLS-DA）, and the related changes in biochemical processes were investigated.Results There were significant differences between the two groups in plasma metabolites,which were the concentration level of VLDL,isoleucine,leucine,valine,LDL,lactic acid,alanine,glycoproteins,glutamine,carnitine,α-ketoglutaric acid, creatine,glycine,unsaturated lipids,tyrosine,methyl histidine,phenylalanine were reduced,while pyruvic acid,citric acid,creatinine,choline,shark inositol,β-glucose,inositol,α-glucose and formic acid level were increased in plasma of patients with papillary thyroid carcinoma,there were significant differences （P〈0.05）compared with the healthy group.Conclusion Based on differences in plasma metabolites we can distinguish differences in plasma metabolites of healthy people and patients with papillary thyroid carcinoma .such metabolomics technology method based ^1 H-NMR provide a reliable metabolism basis for diagnosis of papillary thyroid carcinomale.