维药恰麻古总黄酮对D-半乳糖致衰老小鼠氧自由基代谢的影响

Effects of total flavonoids extract of Brassica rapa L.on metabolism of oxyen free radicals in D-Galactose-induced aging mice

目的探讨维药恰麻古总黄酮对D-半乳糖致衰老模型小鼠氧自由基代谢的影响及其抗衰老作用。方法随机将小鼠分成5组:正常组、模型组、维生素E组及恰麻古总黄酮高、低剂量给药组,除正常组外,其余4组每日上午均颈背部皮下注射D-半乳糖150mg/kg,造模同时,高、低剂量给药组分别灌服恰麻古总黄酮90、30mg/kg,正常组和模型组灌服等容积的生理盐水,采用建立的D-半乳糖致亚急性衰老小鼠模型,同时连续灌服恰麻古黄酮55d。测定小鼠血清、肝脏和脑组织中超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GSH-PX)活性、丙二醛(MDA)的含量。结果恰麻古总黄酮可显著提高D-半乳糖所致衰老小鼠血清、脑和肝脏组织中SOD、GSHPX活力,降低MDA含量,与衰老模型组相比差异有统计学意义(P<0.05)。结论恰麻古总黄酮可通过增强机体清除氧自由基能力,提高抗氧化酶活性而发挥抗衰老作用。

Objective To investigate the effect of total flavonoids extract of Brassica rapa L.on metabolism of oxygen free radicals in D-Galactoseinduced aging mice.Methods The mice were randomly divided into 5 groups：normal group,model group,vitamin E group,high total flavonoids Magu and low dosage group.Except the normal group,the other 4 groups were daily morning neck back subcutaneous injection of D-galactose 150 mg/kg,model at the same time,high,low dose group were intragastrically just total flavonoids Magu 90,30 mg/kg,normal saline group and model group were administrated by volume.Subacute aging model in mice was established by Dgalactose,while continuous irrigation taking just Brassica rapa L.flavonoids in 55 days and activities of SOD and GSH-PX,contents of MDA in the serum,brain, and liver of senile mice were detected.Results Total flavonoids extract of Brassica rapa L.markedly increased the activities of SOD and GSH-PX,significantly decreased contents of MDA in the serum,brain, and liver of aging mice.Conclusion Total flavonoids extract of Brassica rapa L.might have antiaging effect,which is implemented by eliminating oxygen free radicals and rising activities of antioxidases.