红果冬青组培快繁技术研究

Studies on Tissue Culture and Rapid Propagation Techniques of Ilex purpuea Hassk

红果冬青具有较高的药用价值,但传统育苗方式效率低,采用组织培养方法可快速繁殖红果冬青。对红果冬青茎段进行组织培养试验,结果表明:红果冬青最佳灭菌方法为0.1%升汞灭菌8min;最佳启动培养基为MS+BA1.4mg·L-1+NAA0.2mg·L-1,萌芽率可达82.5%;继代培养基1/2MS+BA1.4mg·L-1+NAA0.2mg·L-1+KT0.2mg·L-1,增殖系数达4.1;生根培养基1/2MS+NAA0.2mg·L-1+IAA0.2mg·L-1,生根率达90%。

Ilex purpuea Hassk has high medicinal value, with a low breeding efficiency by traditional style. However, it can be fast bred by the tissue culture method. In this study, Ilex purpuea Hassk stem was obtained for tissue culture test. The results show that the best sterilization time for Ilex purpuea Hassk is 8 min with 0.1% mercuric chloride sterilization; the best initial medium is MS ＋ BA1.4 mg · L^-1 ＋NAA0.2 mg · L^-1 , in which the germination rate can reach 82.5% ; Successive transfer culture medium is 1/2 MS ＋ BA1.4 mg· L^-1 ＋NAA0.2 mg · L^-1 ＋KT0.2 mg ·L^-1, with multiplication coefficient of 4.1. Rooting culture medium is 1/2MS＋NAA0.2 mg · L^-1 ＋IAA0.2 mg · L -1, with rooting rate up to 90%.