HPLC法测定沙棘黄酮粉中槲皮素、山柰素和异鼠李素的含量

HPLC Method in Determining the Content of Quercetin, Kaempferide and Isorhamnetin in Seabuckthorn Flavone Powder

建立同时测定沙棘黄酮粉中槲皮素、山柰素和异鼠李素三种成分含量的HPLC方法,对收集到的7个批次沙棘黄酮粉样品进行质量分析。采用Kromasil C18(4.6mm×250mm,5μm)色谱柱为固定相,甲醇-0.4%磷酸水溶液为流动相,进行梯度洗脱,流速1.0mL/min,检测波长为370nm,柱温25℃。结果显示:该方法稳定可靠,重复性强。收集到的7个批次的沙棘黄酮粉样品中三种成分的含量差异较大,即便是同一厂家不同批次的产品差异也较大。

Objective:To set up a RP-HPLC method to determine the content of Quercetin, Kaempferide and Isorhamnetin in Seabuckthorn flavone powder. Method:The optimum HPLC condition was as follows:Kromasil C18 column(4.6mm×250mm, 5μm)as the stationary phase;Mobile Phase:Methanol-0.4% Phosphoric acid, gradient elution, detection wavelength:370nm;Column Temperature:25℃;Flow Rate:1.0mL/min. Results:The HPLC method was reasonable, stable and accurate. Conclusion:The contents of three components in seven batchs of seabuckthorn flavone powder we collected was quite difference, even in different batchs producted by same company.