松材线虫近交系培育及繁殖力比较

Inbreeding and reproductive comparison of Bursaphelenchus xylophilus

近交系的培育是研究动物遗传学特性的基础。为了研究外来入侵种松材线虫的遗传学基础,选择了中国浙江舟山松材线虫种群和美国宾夕法尼亚州松材线虫种群为研究对象,培育了近交系,并比较了近交系培育前后繁殖力的差异。结果表明:美国宾夕法尼亚州松材线虫种群近交系培育得到8个株系,中国浙江舟山松材线虫种群近交系培育得到4个株系,连续近交对松材线虫的繁殖力未造成显著影响。其中,在近交系培育前后,中国浙江舟山松材线虫种群株系群体繁殖力均优于美国宾夕法尼亚州松材线虫种群。此近交系的培育为进一步研究松材线虫入侵的遗传机制奠定了基础。

The breeding of the inbred line is the basis of studying the characteristics of animal genetics. We studied the genetic basis of alien invasive species-Bursaphelenchus xylophilus, and established the inbred lines of two local populations of China（CN-ZJ） and the United States （US-7H）, and compared the differences of fertility before and after the breeding of the inbred lines. Eight strains were established in the inbreeding of US-7H population and 4 strains were established in the inbreeding of CN-ZJ population. There was no effect of inbreeding on the fecundity of pine wood nematode. The population fecundity of CN-ZJ is better than that of the US-7H population. The breeding of the inbred lines paves the rode for further research about genetic mechanism on the invasion of Bursaphelenchus xylophilus.