母猪粪便中乳酸菌菌株体外益生特性初步研究

A preliminary study on the probiotic characteristics of lactic bacteria strains in vitro from sow feces

试验从健康母猪粪便中分离乳酸菌，并对其益生特性进行初步研究。通过形态和生理生化鉴定，再利用16S rDNA序列同源性分析的方法进行16S rDNA基因扩增与测序，并将测序结果与GenBank中菌株的16S rDNA基因序列进行同源性比较分析，得到1株链球菌，2株约氏乳杆菌，4株乳酸片球菌。体外益生特性结果表明．各菌株在pH2．0～3．0，胆盐浓度0．1％～0．3％N有较强的耐受能力，各菌株对大肠埃希氏菌和单核细胞增多症李氏杆菌均有较好的抑制作用。

In order to gain the lactic acid bacteria as the probiotics and explore the probiotic characteristics,lactic acid bacteria strains were isolated from the sow feces.The morphological and physiological and biochemical identification were determined.Then the genes of I6S rDNA. of these strains were amph6ed and sequenced using the 16S rDNA sequence homology analysis.The sequence results of these strains were compared with other 16S rDNA sequences in GenBank.By Blast assay, 1 strain was Streptococcus sp. ,2 strains were Lactobacillus johnsonii ,and 4 strains were Pediococcus acidilactici.The results showed that probiotic characteristics in vitro showed all strains had higher tolerance to the pH 2.0 - 3.0 and bile concentrations in range of 0.1% - 0.3% and all strains had better inhibitory effect to Escherichia coli and Listeria monocytogenes.