大丽轮枝菌微菌核快速形成培养基的筛选

The Best Medium was Choosed for Fast Cultivation of Verticillium dahliae Microsclerotia

大丽轮枝菌是广泛分布的土壤习居菌,能引起棉花黄萎病害,因其形成的微菌核生命力强,可在土壤中存活多年,是大丽轮枝菌型黄萎病的主要初侵染来源。为开展微菌核的研究,对微菌核形成最佳培养基进行了筛选,采用6种培养基,培养6株大丽轮枝菌(Verticillium dahliae Kleb.),通过生长速率、微菌核数量、微菌核直径和微菌核萌发率4项指标筛选合适的培养基。结果表明:改良燕麦培养基形成微菌核的数量和直径均优于其他培养基;M1和BMM培养基形成微菌核的数量较多,其微菌核直径均小于半组合培养基。因此,改良燕麦、M1和BMM培养基,根据实验需要均可作为快速培养微菌核的理想培养基。

Verticillium dahliae Kleb. is a widely distributed soilborne pathogen causing vascular wilt diseaseson cotton. It is difficult to control for V. dahliae microsclerotia which is the main inoculum. In this study, theoptimum medium of microsclerotium formation and germination were investigated by colony growth rate,microsclerotia quantity, microsclerotia diameter and microsclerotia germination rate. Six different V. dahliae strains collected from Xinjiang were detected. The main results showed that The optimum medium ofmicrosclerotium formation was the oat medium modified, in which the quantity and diameter of microsclerotiawas better than the others types mediums such as PDA, PDA modified, Czapek, M1, BM modified called BMM.The amount of microsclerotium in M1 and BMM was more than the others types mediums, however, thediameter of microsclerotium in them was less than that in semi-defined mediums such as PDA, PDA modifiedand oat medium modified. So, oat medium modified, M1 and BMM were the ideal culture mediums for rapid cultivation of Verticillium dahliae microsclerotia.