一株胶质芽孢杆菌解磷活性及其适宜解磷条件研究

Study on Phosphate-solubilizing Activity and Suitable Conditions of a Strain of Bacillus mucilaginosus

为研究一株胶质芽孢杆菌的解磷活性及适宜解磷的培养条件,在胶质芽孢杆菌的液体培养基中添加难溶性的磷酸钙培养7天后,采用钼锑抗分光光度法测定培养基中可溶性磷的含量,以稀释平板计数法测定菌体浓度,分析培养基中碳源、氯化钠浓度、pH和菌体浓度对解磷活性的影响。结果表明:以葡萄糖作为碳源、0.03%NaCl、pH 6.0时该株胶质芽孢杆菌解磷活性最高,而菌体浓度对解磷活性无显著影响。

In order to study a strain of Bacillus mucilaginosus＇ s phosphate-solubilizing activity and its suitable culture conditions for phosphate-solubilizing, Bacillus mucilaginosus was cultured for 7 days in the liquid medium added with lowly soluble calcium phosphate. The content of soluble phosphate in the medium was determined by the means of Molybdenum-Antimony Anti-Spectrophotometric method, and the concentration of cultured cell was measured using dilution plate counting. The effects of carbon source, NaC1 concentration, pH value and cell concentration on phosphate-solubilizing activity were analyzed. The results showed that, the phosphate-solubilizing activity of Bacillus mucilaginosus reached the peak when the with the glucose （as carbon source） and 0.03% NaC1 in a pH value of 6.0. However the no significant effects on phosphate-solubilizing activity. medium was supplied cell concentration had