OK-432肿瘤细胞疫苗对DBA/2小鼠舌癌生长抑制作用

OK-432 tumor cell vaccine inhibits tongue cancer growth in DBA/2 mice

目的利用DBA/2小鼠移植舌癌荷瘤模型,探究OK-432肿瘤细胞疫苗对舌癌的抑制作用。方法以戊二醛为交联剂,使KLN-205细胞与OK-432产生交联,制备OK-432肿瘤细胞疫苗;将DBA/2小鼠随机分为4组,每组20只,分别右侧腹部皮下注射0.9%氯化钠注射液、OK-432、灭活的KLN-205细胞、OK-432疫苗,接种KLN-205肿瘤细胞后观察对比舌癌瘤体大小,并用统计学分析其结果。结果肿瘤大小除KLN-205组与对照组间差异没有统计学意义(P=0.094)外,其余各组间差异均有统计学意义,其中OK-432疫苗组肿瘤体积均小于其他3组。结论 OK-432肿瘤细胞疫苗能抑制小鼠舌癌的生长,具有一定的抗肿瘤效应。

Objective To investigate the inhibitory effects of OK-432 tumor cell vaccine on tongue cancer growth in DBA/2 mice by using the tongue cancer xenograft models. Methods The OK-432 tumor cell vaccine was prepared by inducing conjugation of OK-432 to squamous cell carcinoma （KLN-205） with 0.2% glutaraldehyde （GA）. The DBA/2 mice were randomly allocated to receive subcutaneous injection of saline （ n=20）, OK-432 （n=20）, inacti-vated KLN-205 cells （n=20） and OK-432 tumor cell vaccine （n=20） on the right abdomen. This allowed assessment of the tumor size before and after KLN-205 cell inoculation. Results The tumor size was significantly different among the four groups except for OK-432 vaccine group compared with control group （P=0.094）. Inoculation with OK-432 vaccine resulted in marked reduction in the tumor size compared with the remaining three groups. Conclusion OK-432 tumor cell vaccine can inhibit the growth of tongue cancer in mice, suggesting the potential anti-tumor effects.