QuEChERS/UPLC-MS测定火锅调料中罂粟壳(粉)的研究

Determination of Shells and Powder of Papaver somniferum L. in Chafing Dish Seasoning by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry via QuEChERS

目的建立检测火锅调料中非法添加罂粟壳（粉）的方法。方法样品经QuEChERS方法提取、净化后，采用超高效液相色谱串联三重四极杆质谱仪（UPLC．MS），在多反应监测（MRM）模式下，通过色谱、质谱条件优化及方法学考察，建立了高效、准确检测火锅调料中非法添加罂粟壳（粉）的方法。结果本方法的线性范围为：那可丁2．470～49．405ng／mL，r=0．9994；罂粟碱2．090～41．804ng／mL，r=0．9995；蒂巴因2．042~40．840ng／mL，r=1．000；可待因11．287～225．733ng／mL，r=0．9996；吗啡10．420～208．400ng／mL，r=0．9999。那可丁、罂粟碱、蒂巴因、可待因、吗啡的检测限分别为0．21，0．33，0．31，10．37，4．11μg／kg。回收率78．4％～100．6％。结论本方法快速、准确、灵敏度高，可用于检测火锅调料中的罂粟壳（粉）。

Objective To establish a method for determination of shells and powder of Papaver somniferum L. in hot pot seasoning. Methods After extraction and purification by QuEChERS, ultra performance liquid chromatography- tandem mass spectrometry （UPLC-MS） were used to determine the shells and powder of Papaver somniferum L. in hot pot seasoning by MRM mode using internal standard. The analysis method was established by optimization of parameters of UPLC/MS and verification test. Results The linear ranges were as follows： 2.470-49.405 ng/ mL（r=0.9994） for narcotine, 2.090-41.804 ng/mL（r=0.9995） for papaverine, 2.042-40.840 ng/mL （r=l.000） for thebaine, 11.287-225.733 ng/mL（r=0.9996） for codeine, 10.420-208.400 ng/mL（r=0.9999） for morphine. The determination limits were as follows： 0.21 gg/kg for narcotine, 0.33 gg/kg for papaverine, 0.31 μg/kg for thebaine, 10.37 μg/kg for codeine, 4.11 μg/kg for morphine. The average recoveries were 78.4 %-100.6 %. Conclusion This method is quick, accurate and sensitive, and can be widely used for determination of shells and powder of Papaver somniferum L. in hot pot seasoning.