朱日很滴丸对大鼠急性心肌缺血的影响

Effect of Zhurihen dripping pills on acute myocardial ischemia in rats

目的观察朱日很滴丸是否对垂体后叶素诱导的大鼠急性心肌缺血有保护作用。方法将大鼠随机分为正常对照组,模型组,丹参滴丸阳性对照组,朱日很滴丸高、中、低剂量组,连续给药10d,采用大鼠颈静脉注射垂体后叶素注射液(3.75mg/kg)造成急性心肌缺血模型,记录各组大鼠给予垂体后叶素前后的心电图变化,同时测定血清及心脏组织匀浆上清液的乳酸脱氢酶(LDH)、肌酸激酶(CK)含量。结果朱日很滴丸能够显著增加垂体后叶素引起的5s时心率的降低,300s时T波幅度的降低;300,1 500mg/kg朱日很滴丸能显著降低模型大鼠ΔST变化幅度;低、中、高剂量组无明显的量效关系;各组大鼠血清、心脏组织匀浆CK、LDH含量没有显著差异。结论朱日很滴丸能显著改善急性心肌缺血大鼠的心电图异常,量效关系不明显。

Objective To investigate whether Zhurihen dripping pills has a protective effect on vasopressin induced myocardial ischemia .Methods The rats were randomly divided into normal control group ,model group , positive control group with compound Danshen dripping pill ,high ,medium and low dose of zhurihen dripping pill group.Administration for 10 days ,the rat were caused acute myocardial ischemia model by injection of vasopressin injection liquid （3 .75 mg/kg） through jugular vein ,the determination of serum ECG changes and lactate dehydro-genase （LDH） ,creatine kinase （CK） levels of heart tissue homogenate were recorded and determined simultane-ously for the rats given vasopressin before and after administration .Results The Zhurihen dripping pill can signif-icantly increase heart rate reduced in 5 s by pituitrin ,the reduction was on the amplitude of T wave in 300 s;300 , 1 500 mg/kg Zhurihen dripping pill can significantly reduce ΔST amplitude of variation in the model rats .No obvious dose effect relationship was not found in the low ,middle and high dose groups .There were no significant difference for serum heart homogenate CK and LDH in each group of rats.Conclusion The zhurihen dripping pill can significantly improve the abnormal ECG of acute myocardial ischemia in rats ,and the dose effect relationship is not obvious.