六味顺激胶囊对肝郁脾虚型肠易激综合征模型大鼠内脏敏感性及五羟色胺水平的影响

Effects of Liuwei Shunji Capsule on Visceral Hypersensitivity and Content of 5-HT of Rats with Liver Depression and Spleen Deficiency-type Irritable Bowel Syndrome

目的观察六味顺激胶囊对肝郁脾虚型肠易激综合征(IBS)大鼠的生存状态、内脏敏感性、五羟色胺(5-HT)水平的影响,探讨其作用机制。方法 SD大鼠随机分为正常组、模型组、阳性药组和六味顺激胶囊高、中、低剂量组,采用番泻叶结合束缚的方法复制肝郁脾虚型IBS大鼠模型,造模后第2日,各给药组给予相应药物,每日1次,连续2周。观察肝郁脾虚型IBS大鼠生存状态、内脏敏感性,测定血清及下丘脑5-HT含量。结果与模型组比较,六味顺激胶囊高、中、低剂量组大鼠体质量增加(P<0.05),泄泻、神态倦怠、皮毛散乱、脾气暴躁、易怒等症状改善,内脏敏感性显著降低(P<0.01),血清及下丘脑5-HT含量显著降低(P<0.01)。结论六味顺激胶囊能显著改善肝郁脾虚型IBS模型大鼠的生存状态及肠道功能紊乱状态,其可通过调节大鼠5-HT水平发挥治疗作用。

Objective To observe the therapeutic effects of Liuwei Shunji Capsule on survival state, visceral hypersensitivity, the content of 5-HT of model rats with liver depression and spleen deficiency-type irritable bowel syndrome （IBS）, and explore its mechanism.Methods SD rats were randomly divided into normal group, model group, pinaverium bromide group and Liuwei Shunji Capsule of high, medium and low dose groups. Senna with restraint stress was used in duplicating liver depression and spleen deficiency-type IBS model. From the second day after the model was established, each drug treatment group was administered with corresponding drugs once a day for two weeks. The effects of Liuwei Shunji Capsule on survival state, visceral hypersensitivity, the content of 5-HT in serum and hypothalamus were observed.Results Compared with model group, Liuwei Shunji Capsule could raise weight growth rate of model rats with liver depression and spleen deficiency-type IBS （P〈0.05）, improve their diarrhea, fatigue demeanor, fur quality, irritability and other symptoms, significantly decrease their visceral hypersensitivity, the content of 5-HT in serum and hypothalamus （P〈0.01）.Conclusion Liuwei Shunji Capsule can effectively improve survival state and intestinal disorders of rats with liver depression and spleen deficiency-type IBS, and the mechanism of treatment could be realized through regulating 5-HT level.