高脂高糖饲料对建立实验糖耐量受损大鼠模型的效果分析

Analysis of the effect of high glucose and high fat diet on the manufacturing of the experimental pre-diabetic rats model

目的探讨脂肪和蔗糖对诱导大鼠糖耐量受损的作用及影响因素,并分析产出指标间的相互关系。方法选择两批不同体重的Wistar大鼠,分别随机分为4组(第2批)和5组(第1批),包括对照组和不同配比的高脂高糖饲料(HFS)组,每组至少10只。喂养8周后,通过测定体重、血糖和血脂变化,并采用多因素分析,比较脂肪、糖水平对引发大鼠糖耐量受损指症的影响。结果与对照组相比,高脂高糖饲料组大鼠体重显著增加,血糖、血脂明显升高,其增加幅度与鼠龄负相关(P<0.05)。多因素分析结果显示:脂肪是引起大鼠体重增长、血糖、血脂异常的主要因素(P<0.05),高脂高糖饲料引起的血糖耐量受损与脂肪摄入量的关系具有明显的统计学意义。高糖饲料对血糖的影响主要起到辅助增高的作用,但却可明显升高TG水平(P<0.05),随着饲料中蔗糖水平增高TG上升趋势明显。结论制造糖耐量受损实验大鼠模型时,应尽量选择低龄大鼠;高脂高糖饲料中的蔗糖和脂肪配比要合理,以10%脂肪、20%蔗糖的比例较好。

Objective To evaluate the roles of fat and sugar in inducing pre-diabetes rats and their effect factors, and analyze the relationship between the indicators. Methods Two batches of Wistar rats were randomly divided into 4 and 5 groups. The groups were control groups fed by maintain feed and groups fed by different proportions of high fat and sugar feed （HFS）. After dietary intervention for eight weeks, body weight, blood glucose and lipid levels of the rats were measured. The effect of different fat and sugar levels in the feed on pre-diabetic rats were compared using multi-factor analysis. Results Compared with the control group, the body weight of Wistar rats fed by HFS feed increased significantly, and so did the blood glucose and blood lipids. The extent of increase was negatively correlated with rat age （P 〈 0.05）. Using multivariate analysis, it is found that fat was a major factor in causing rats＇ weight gain, abnormal blood glucose and lipid （P 〈 0. 05 ）. High sugar feed mainly played an auxiliary role in increasing blood glucose, while it could significantly increase the level of TG （P 〈 0.05）. As the sucrose levels of dietary increased the TG rose significantly. Conclusion When the prediabetes rat model was built, younger rats should be chose. The diet with 20% fat content had the greatest effect on the blood glucose of the rats and the diet with 20% sucrose increased the blood triglyceride concentrations of the rats significantly.