高效液相色谱法检测化妆品中5种限用防腐剂

Determination of five kinds of restricted preservatives in cosmetics by HPLC

目的开展化妆品中5种限用防腐剂（氯己定、苯甲酸、氯二甲酚、O-苯基苯酚和p-氯-m-甲酚）检测方法的研究。方法以甲醇提取市售10种化妆品中5种防腐剂组分，饱和氯化钠溶液作为沉淀剂，以Agilent Eclipse XDB-C18（250mm×4．6mm，5μm）为色谱分析柱，甲醇-0．1mol／L磷酸二氢钠水溶液（pH3．0）为流动相洗脱，流速为1．0ml／min，检测波长为285nm进行测定。结果氯己定等5种防腐剂在一定浓度范围内呈良好线性，相关系数均大于0．9990，检出限（LOD）为0．8～2．2ng，方法的回收率为95．0％～104．0％。结论该方法准确可靠，是对规范方法有益的补充。

Objective In order to detect and monitor preservative component in cosmetic, the method for determination of five kinds of restricted substances benzoic acid, chloroxylenol,o-phenyl phenol and p-chloro-m-cresol chlorhexidine in cosmetics by HPLC was carried out. Methods 10 kinds of commercial cosmetics as test samples, using methanol as the extracting solution, saturated sodium chloride solution as precipitant, and Agilent Eclipse XDB-Cx8 （250 mm × 4.6 mm, 5 μm） as analytical column methanol -0. 1 mol/L sodium dihydrogen phosphate solution （pH3.0） was as mobile phase eluting at a flow rate 1.0 ml/min, the detective wavelength was 285 nm. Results The method for determination of chlorhexidine and other four kinds of restricted substances had good linear relationship, the linear correlation coefficients were greater than 0. 9990, the limit of detection （LOD） of 0. 8 - 2.2 ng, the average recoveries were between 95.0% - 104.0%. Conclusion The method is accurate and reliable, and it is a useful supplement for ＂Hygienic Standard for Cosmetics＂