应用实时荧光定量技术评价粉红粘帚霉对水稻纹枯病的田间防治效果

Assessment the biocontrol efficacy of Gliocladium roseum on rice sheath blight using Real-time fluorescent PCR method

本研究利用实时荧光定量PCR技术监测施用生防菌前后土壤中粉红粘帚霉67-1和水稻纹枯病菌AG-IIA的数量,并调查发病情况,收割后测产,评估生防菌粉红粘帚霉67-1的田间防治效果。实时荧光定量检测结果表明,粉红粘帚霉67-1防治水稻纹枯病的效果随着用药浓度的增加而增强,各处理间差异极显著。用药后土壤中粉红粘帚霉67-1的含量在一定时间内随培养时间的延长逐渐升高,停止用药后逐渐减少。水稻纹枯病菌在清水对照组中没有显著变化,在处理组中水稻纹枯病菌的数量随时间降低。60 g/667 m2菌量的防效及产量与井冈霉素对照组差异不显著。防病保产结果与实时荧光定量结果基本一致,说明粉红粘帚霉67-1的孢子制剂对水稻纹枯病具有优良的田间防治效果,实时荧光定量PCR技术可以用于生物防治效果的田间评价。

Gliocladium is important biocontrol microbe and the protection and therapy Gliocladium roseum 67-1 had been proved. This paper aimed at evaluation field efficacy of 67-1 protect and control rice sheath blight of G. roseum 67-1 and Rhizoctonia solani AG-1IA by the method of Realtime fluorescent quantitative PCRdisease indexand yield. The Real-time PCR results found that the numbers of 67-1 increased with time, the highest quantity at the 22th day and then decreased. Additionally, the numbers of rice sheath blight pathogen A-1IA no significant change in the pure water, and data of 60g/667m^2 treatment had no obviously difference with that of validamycin A control. Field trial results showed that G. roseum 67-1 had obvious of protect and therapy, which increase the yield of rice. In conclusion, the G. roseum 67-1 spore was effective in controling rice sheath blight pathogen, and Real-time PCR could be used for evaluation of efficacy of biocontrol agents.