摘要
本研究利用实时荧光定量PCR技术监测施用生防菌前后土壤中粉红粘帚霉67-1和水稻纹枯病菌AG-IIA的数量,并调查发病情况,收割后测产,评估生防菌粉红粘帚霉67-1的田间防治效果。实时荧光定量检测结果表明,粉红粘帚霉67-1防治水稻纹枯病的效果随着用药浓度的增加而增强,各处理间差异极显著。用药后土壤中粉红粘帚霉67-1的含量在一定时间内随培养时间的延长逐渐升高,停止用药后逐渐减少。水稻纹枯病菌在清水对照组中没有显著变化,在处理组中水稻纹枯病菌的数量随时间降低。60 g/667 m2菌量的防效及产量与井冈霉素对照组差异不显著。防病保产结果与实时荧光定量结果基本一致,说明粉红粘帚霉67-1的孢子制剂对水稻纹枯病具有优良的田间防治效果,实时荧光定量PCR技术可以用于生物防治效果的田间评价。
Gliocladium is important biocontrol microbe and the protection and therapy Gliocladium roseum 67-1 had been proved. This paper aimed at evaluation field efficacy of 67-1 protect and control rice sheath blight of G. roseum 67-1 and Rhizoctonia solani AG-1IA by the method of Realtime fluorescent quantitative PCRdisease indexand yield. The Real-time PCR results found that the numbers of 67-1 increased with time, the highest quantity at the 22th day and then decreased. Additionally, the numbers of rice sheath blight pathogen A-1IA no significant change in the pure water, and data of 60g/667m^2 treatment had no obviously difference with that of validamycin A control. Field trial results showed that G. roseum 67-1 had obvious of protect and therapy, which increase the yield of rice. In conclusion, the G. roseum 67-1 spore was effective in controling rice sheath blight pathogen, and Real-time PCR could be used for evaluation of efficacy of biocontrol agents.
出处
《植物病理学报》
CAS
CSCD
北大核心
2014年第4期422-427,共6页
Acta Phytopathologica Sinica
基金
国家自然科学基金项目(30971954)
江苏高校优势学科建设工程资助项目