摘要
[目的]通过对不同采收期老翘内连翘苷、连翘酯苷A、浸出物的含量测定,为老翘适宜采收期的确定提供科学依据。[方法]采用HPLC法测定不同采收期老翘果实中连翘苷、连翘酯苷A的含量;采用《中国药典》2010版一部(附录ⅩΑ)项下醇溶性浸出物冷浸法测定;以Luna5uC18(2)100A(250 mm×4.6 mm,5μm)为色谱柱,连翘苷测定流动相为乙腈-水(25∶75,V∶V),检测波长为277 nm,柱温为室温,流速为1.0 mL/min;连翘酯苷A测定流动相为乙腈-0.4%冰醋酸溶液(15∶85,V∶V),检测波长为330 nm,柱温为室温,流速为1.0mL/min。[结果]连翘苷在3.33~13.32μg范围内线性关系良好(R2=0.999 7),平均回收率为98.9%,RSD=1.8%;连翘酯苷A在4.17~6.69μg具有良好的线性关系(R2=0.999 7),平均回收率为101.7%,RSD=0.9%。[结论]连翘苷、连翘酯苷A、浸出物在老翘整个采收期试验过程中都呈现不断下降的趋势,其中连翘苷的含量测定结果在老翘整个采收期试验过程中都达不到2010版药典的规定标准,连翘酯苷A、浸出物的含量结果分别在2013年1月20日、2月25日采集的样品测定结果中达不到2010版药典的规定标准。
[Objective] The aim was to provide a scientific basis for determination of harvest time of the old alice, through the determination of forsythin, forsythoside A and extract in old alice with different harvest time.[Method] HPLC method was used for analyzing the contents of forsythin and forsythiaside A in different harvest time of old alice, and multi-speed oscillator was used for analyzing the contents of extract. Luna5uC18(2) 100A(250 mm ×4.6 mm, 5 μm) column was carried out and the mobile phrase of determination forsythin was acetonitrile-water(25∶75, V∶V) and the detection wavelength was 277 nm.The mobile phase of determination forsythiaside A was acetonitrile-0.4% acetic acid(15∶85, V∶V)and the detection wavelength was 330 nm.The column temperature was room temperature. And the flow rate was 1.0 mL/min.[Results] There was a good linear relationship(R2=0.999 7) for forsythin at 3.33-13.32 μg and the average recovery rate was 98.9%,and RSD was 1.8%.The good linear relationship(R2=0.999 7) was for forsythiaside A at 4.17-6.69 μg and the average recovery rate was 101.7% and RSD was 0.9%. [Conclusion] Forsythin, forsythiaside A and extract were decreased during the whole experiment have shown a declining trend, in which the content of phillyrin in the whole experiment were not up to the required 2010 version Pharmacopoeia standards. Forsythiaside A and extract respectively in January 20 th, 2013 and February 25 thwould not meet the required standard Pharmacopoeia 2010 edition.
出处
《园艺与种苗》
CAS
2014年第4期44-47,共4页
Horticulture & Seed
基金
国家"十二五"科技支撑项目(2011BA107B01)
山西省科技攻关项目(20120313015-9)
长治市科技计划项目(20118033-1)
关键词
老翘
不同采收期
连翘苷
连翘酯苷A
浸出物
Old alice
Different harvest
Forsythin
Forsythoside A
Extract
HPLC