葛枣猕猴桃叶片蛋白质双向电泳体系的研究

Study of Two-dimensional Electrophoresis Platform for Actinidia polygama Leaf Proteomic

[目的]建立葛枣猕猴桃叶片蛋白质双向电泳体系。[方法]通过对不同的蛋白质提取方法、裂解液配方及IPG胶条的探索,建立适应于葛枣猕猴桃叶片双向电泳技术体系。[结果]TCA/丙酮法+配制裂解液较适用于葛枣猕猴桃叶片蛋白质组分析。最终可获得蛋白质点丰富、分辨率高的双向电泳图谱,可以检测出450个清晰的蛋白质点。[结论]该研究为在蛋白质组水平揭示叶片颜色变化的调控机制奠定基础。

[Objective] The aim was to establish the two-dimensional electrophoresis platform of Actinidia polygama leaf.[Method] Different protein extraction method,cracking fluid formulation and length of the gel strips were tested.[Result] The result showed that the manual confect cracking fluid formulation was suitable for the leaf protein dissolution than the sequential extraction kit.450 protein spots were detected and the high-resolution two-dimensional electrophoresis map was got.[Conclusion] The study laid foundation for revealing regulatory mechanism of leaf color changes in proteomics level.