TPIN对HepG2肝癌细胞生物学特性的影响

Effect of TPIN on Cytobiology Characteristics of HepG2 Hepatoma Carcinoma Cells

目的研究重组减毒沙门菌菌株TPIN对HepG2细胞生物学性能的影响。方法 TPIN转染HepG2细胞36 h时收集细胞表达上清,MTT法检测表达产物对HepG2细胞生长的作用;Transwell迁移实验观察TPIN表达产物对HepG2细胞迁移的影响;将不同剂量表达产物分别加入甲基纤维素小碟,并将小碟小心放入鸡胚绒毛尿囊膜,37.8℃继续孵化3 d,观察IL-2/NK4表达产物体外对鸡胚绒膜尿囊膜血管的效应。结果 TPIN表达上清显著抑制HepG2细胞增殖的活性,且有一定剂量依赖效应;表达产物可明显抑制肿瘤细胞迁移,且明显抑制鸡胚绒膜尿囊膜血管形成。结论 TPIN在体外能高效转染肿瘤细胞,表达目的蛋白IL-2及NK4,且表达产物能够抑制HepG2细胞的增殖和迁移,对鸡胚绒膜尿囊膜血管的形成有抑制效应。

Objective To study the effect of an recombinant of attenuated Salmonella typhimurium strain （TPIN） on cytobiology characteristics of HepG2 hepatoma carcinoma cells. Methods Cultured supernatant was collect-ed from TPIN-transfected HepG2 cells for 36 h, and the effect of expression products on HepG2 cell growth was detected by MTT assay; the effect of TPIN expression products on HepG2 cell migration was assessed by Transwell method; differ-ent dosages of expression products were put on the Methylcellulose small plates, and then the small plates were put into chick chorioallantoic membrane and was hatched at 37. 8℃ for 3 d, and then angiogenic effect of different dosages of IL-2 / NK4 expression products on chick embryo was observed. Results Supernatant collected from TPIN-transfected cells significantly inhibited the activity of HepG2 cell multiplioation with a certain dose-dependent effect; expression products significantly inhibited the cell migration as well as the angiogenesis in chick chorioallantoic membrane. Conclusion TPIN can effectively transfect HepG2 cells in vitro and express IL2 and NK4 stably, and expression products can inhibit HepG2 proliferation and migration as well as the angiogenesis inhibition of chick chorioallantoic membrane.