氟络草酮染毒对成年大鼠睾丸的影响

Oral exposure of fluorochloridone caused testes damage of sparague dwaley rats

目的 目的探讨除草剂氟络草酮（fluorochloridone,FLC）对SD大鼠睾丸的影响.方法 40只SD雄性大鼠随机分为对照组和30、150、750 mg/kg氟络草酮染毒组,每组10只.FLC灌胃染毒28 d,1次/d.用分光光度法检测睾丸氧化应激水平,用光学显微镜及电子显微镜观察睾丸组织病理变化,计数附睾尾精子数,评价FLC的睾丸毒性.结果 750 mg/kg染毒组睾丸质量及脏器系数、附睾质量和附睾尾精子计数明显低于对照组,差异均有统计学意义（P＜0.05或P＜0.01）.与对照组比较,各染毒剂量组谷胱甘肽（GSH）含量、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活力均明显下降,差异有统计学意义（P＜0.05或P＜0.01）.150和750 mg/kg染毒组谷胱甘肽过氧化物酶（GSH-Px）、谷胱甘肽-S转移酶（GSH-ST）活力明显下降,丙二醛（MDA）含量明显升高,与对照组比较,差异均有统计学意义（P＜0.05或P＜0.01）.750 mg/kg染毒组谷胱甘肽还原酶（GSH-GR）活力明显降低,与对照组比较,差异有统计学意义（P＜0.05）.光学显微镜及电子显微镜观察显示,150、750 mg/kg染毒组睾丸生精上皮退行性改变,Sertoli细胞空泡化,生精细胞脱落,细胞核损伤.结论 FLC通过改变氧化应激水平,引起大鼠睾丸损伤.

Objective To investigate the effects of fluorochloridone （FLC） exposure on the testes of adult Sprague-Dawley （SD） rats.Methods Forty male SD rats were randomly divided into four groups.These groups,each of 10 male rats,were separately given FLC by gavage at a dose of 0 （control）,30,150,or 750 mg/kg once daily for 28 d.The oxidative stress biomarkers in the testes were measured by spectrophotometry.The pathological changes in testicular tissues were evaluated under the light and electric microscopes.The cauda epididymal sperm count was determined.The testicular toxicity of FLC was assessed accordingly.Results Compared with the control group,the 750 mg/kg FLC group had significantly lower testicular weight and organ coefficient,epididymal weight,and cauda epididymal sperm count （P〈0.05 or P〈0.01）,the 150 and 750 mg/kg FLC groups had significantly increased malonaldehyde content （P〈0.05 or P〈0.01）,each exposed group had a significantly reduced glutathione （GSH） level （P〈0.05 or P〈0.01）,the 750 mg/kg FLC group had significantly reduced activities of superoxide dismutase （SOD）,catalase （CAT）,GSH peroxidase,GSH S-transferase （GSHST）,and GSH reductase （GSH-GR） （P〈0.05 or P〈0.01）,the 150 mg/kg FLC group showed significant decreases in the activities of all antioxidant enzymes except GSH-GR （P〈0.05 or P〈0.01）,and the 30 mg/kg FLC group showed significant decreases in the activities of SOD and CAT （P〈0.05 or P〈0.01）.Furthermore,seminiferous epithelial degeneration,Sertoli cell vacuolization,spermatogenic cell loss,and nuclear damage were observed under the light and electronic microscopes in the 150 and 750 mg/kg FLC groups.Conclusion FLC could damage the testes of adult rats by inducting oxidative stress.This research provided clues and directions for further exploration of the mechanism of FLC testicular toxicity.