TNF-α和IFN-γ诱导人乳头状甲状腺癌细胞FAT10过表达及染色体核型异常

TNF-α /IFN-γ induced FAT10 expression and abnormal chromosome karyotype in TPC-1 cells

目的:观察TNF-α和IFN-γ通过诱导人乳头状甲状腺癌TPC-1细胞中FAT10的表达而导致染色体核型异常。方法:采用电穿孔法将FAT10 siRNA转染人TPC-1细胞,采用Western blot检测各组细胞中FAT10蛋白的表达变化,用染色体核型分析TNF-α和IFN-γ诱导TPC-1细胞非整倍体现象。结果:Western blot结果显示,TPC-1细胞经TNF-α和IFN-γ诱导后,FAT10过表达。FAT10 siRNA干扰可以有效抑制TPC-1细胞中FAT10的表达,并且通过抑制FAT10表达,可以有效抑制TNF-α和IFN-γ诱导的非整倍体。进而发现FAT10表达具有拮抗TNF-α和IFN-γ诱导的TPC-1细胞凋亡的作用。结论:FAT10介导了TNF-α和IFN-γ诱导的TPC-1细胞的非整倍体现象,并具有拮抗凋亡的作用。

Objective：To observe the FAT10 protein expression and abnormal chromosome karyotype in TPC 1 papillary thyroid carcinoma cells after TNF αand IFN γ stimulation. Methods ： Transfection of siRNA into TPC 1 cells used the electroporation system. Western blot was employed to detect the expression levels of FAT10 protein in TPC 1 cells after transfection. Cytogenetic analysis was performed to analyze the chromosome numbers. Results：The TNF α and IFN γ induced over expression of FAT10 gene was significantly inhibited after FAT10 siRNA trans fection. At the same time, TNF α and IFN γ/induced abnormal chromosome karyotype were also blocked. Conclu sion： siRNA interference inhibits the expression of FAT10 gene and TNF α induced abnormal chromosome karyotype in TPC 1 cells.