过表达Axin对胶质瘤细胞U87的生物学行为的影响及其作用机制

The effect of over-expression of Axin on the biological behavior of glioma U87 cells and its mechanism

目的:观察Axin对胶质瘤U87细胞生物学行为的影响,并探讨其机制。方法:pCMV5-HA-Axin瞬时转染胶质瘤U87细胞,TUNEL法检测细胞的凋亡,流式细胞仪检测瘤细胞周期变化,Western blot检测Axin及β-catenin蛋白质的表达。结果:Axin显著促进胶质瘤U87细胞的凋亡、抑制细胞的增殖;促进β-catenin蛋白在胞浆内的蓄积。结论:过表达Axin的胶质瘤U87细胞可能通过降低细胞核内β-catenin的蛋白表达量抑制Wnt信号通路,从而抑制细胞增殖并促使细胞凋亡。

Objective： To investigate the effect of Axin on the biological behavior of U87 cells of glioma and to elu cidate its molecular mechanism. Methods： U87 cells of glioma were transfered with pCMV5 HA Axin. The cell proliferation was detected by flow cytometry and the apoptosis was analyzed by TUNEL assay. At the same time, Western blot was used to detect the expression of Axin and β catenin in U87 cells. Results： Over expression of Ax in inhibited significantly the growth of U87 cells. Axin increased the proportion of cytoplasmic β catenin. Conclu sion： Over expression of Axin might inhibit the activity of Wnt signaling pathway by decreasing nuclear β catenin, and its over expression can inhibit the proliferation and induce the apoptosis of U87 ceils.