泽泻提取物对大鼠脑缺血-再灌注损伤氧化应激的影响

Effect of Rhizoma Alismatis extracts on oxidative stress induced by cerebral ischemia-reperfusion injury in rats

目的 观察泽泻提取物对大鼠局灶性脑缺血再灌注损伤中氧化应激的影响及作用机制. 方法 随机将雄性SD大鼠分为假手术组、模型组、泽泻提取物组及尼莫地平阳性对照组.灌胃14 d后采用线栓法制备大鼠局灶性脑缺血-再灌注损伤模型,24 h后对其神经功能障碍进行评分,测定梗死范围和计算脑含水量,检测血清和脑组织中丙二醛（MDA）、超氧化物歧化酶（SOD）、一氧化氮（NO）含量及诱导型一氧化氮合酶（iNOS）活性. 结果 与模型组神经功能评分（2.78±0.43）分、脑组织含水量（88.33±4.15）％、脑梗死面积（0.35±0.04）％、血清MDA（8.21±1.47）μmol/L、SOD（75.52±20.78）mU/L、NO（41.73±3.85） μmol/L、iNOS（23.49±3.53）mU/L、脑组织中MDA（7.58±1.49）μmol/L、SOD（150.11±13.62） mU/L、NO（22.08±1.57）μmol/L、iNOS（13.28±1.84）mU/L比较,泽泻提取物组大鼠神经功能障碍减轻,脑含水量明显降低,脑梗面积缩小,血清和脑组织中的MDA、NO含量及iNOS活性均显著降低,SOD活性显著升高[（2.2l±0.38）分、（81.18±2.09）％、（0.26±0.07）％、（5.92±1.64） μmol/L、（1 15.48±18.65）mU/L、（28.23±4.32）μmol/L、（15.31±1.68）mU/L、（5.41±0.68）μmol/L、（168.57±10.65） mU/L、（14.37±0.77） μmol/L、（9.83±0.75）mU/L,均P＜0.05]. 结论 泽泻提取物对局灶性脑缺血-再灌注损伤具有保护作用,可能与抗氧化和清除自由基有关.

Objective To investigate the effects of Rhizoma Alismatis extracts on oxidative stress induced by cerebral ischemia-reperfusion injury in rats,and to explore its protective mechanism in cerebral ischemia-reperfusion injury.Methods A total of 60 male SD rats were randomly divided into sham operation group,model group,Alisma orientalis group and Nimodipine positive control group （n=15,each）.Cerebral ischemia-reperfusion injury model was prepared by suture method after 14 days of intragastric administration.After 24 hours,scores of neurological dysfunction,the infarct size,the water content of the brain,the malondialdehyde （MDA）,superoxide dismutase （SOD）,nitric oxide （NO） levels in serum and brain tissues,and the activity of inducible NO synthase （iNOS）were detected.Results As compared with the model group,Alisma orientalis group showed that the scores of neurological dysfunction,cerebral water content,cerebral infarction size,contents of MDA and NO,and the activity of iNOS were significantly reduced,and the activity of SOD was significantly increased in respectively [（2.21 ± 0.38） vs.（2.78 ± 0.43）,（81.18 ± 2.09）％ vs.（88.33±4.15）％,（0.26±0.07） ％ vs.（0.35±0.04）％,（5.92±1.64） μmol/L vs.（8.21±1.47）μmol/L,（115.48±18.65） mU/L vs.（75.52±20.78） mU/L,（28.23±4.32） μmol/L vs.（41.73±3.85） μmol/L,（15.31±1.68） mU/L vs.（23.49±3.53） mU/L,（5.41±0.68） μmol/L vs.（7.58±1.49） μmol/L,（168.57±10.65） mU/L vs.（150.11±13.62） mU/L,（14.37±0.77） μmol/L vs.（22.08±1.57） μmol/L,（9.83±0.75） mU/L vs.（13.28±1.84） mU/L,respectively,all P＜0.05]Conclusions Alisma orientalis extract has the protective effect on focal cerebral ischemia reperfusion injury,and the mechanism may be related to antioxidant and scavenging free radicals.