老年豚鼠喉部细胞外基质成分的改变和意义

The changes and significance of the extracellular matrix in larynx of old guinea pig

目的 探讨老年豚鼠喉部透明质酸和胶原蛋白的变化及意义. 方法 选取健康4月龄（对照组）豚鼠和2年龄豚鼠（老年组）各6只,分别用Alcian blue和Masson三色染色法观察两组豚鼠喉部透明质酸和胶原蛋白的表达情况,用Image-Pro Plus 6.0 For Window图像分析软件处理,对两组结果进行比较. 结果 对照组透明质酸和胶原蛋白表达的吸光度值分别为0.80和0.63,而老年组分别为0.69和0.71,老年组透明质酸的含量较对照组降低,胶原蛋白含量较对照组升高,差异均有统计学意义（F均分别为13.45、9.56,均P＜0.05）. 结论 老年豚鼠喉部透明质酸降低,胶原蛋白增加,可能通过改变声带的黏弹性能导致嗓音发生年龄相关性改变.

Objective To study the changes and significance of the hyaluronic acid （HA） and collagen in the larynx of old guinea pig.Methods Six young guinea pigs （4 months old） and 6 old guinea pigs （2 years old） were selected as control group and aged group for this study （n=6 for each）The expressions of HA and collagen in the larynx of guinea pigs were detected using Alcian blue and Masson trichrome methods,respectively.The images were managed using Image-Pro Plus 6.0 For Window.Results The optical density （expression level） of HA in the larynx was lower in aged group than in control group （0.69 vs.0.80,F=13.45,P＜0.05）.The optical density（expression level） of collagen in the larynx was higher in aged group than in control group （0.71 vs.0.63,F=9.56,P＜0.05）.Conclusions The content of HA in larynx is decreased and collagen in larynx is increased in old guinea pig which may result in presbyphonia change through higher viscoelastic properties of vocal cords.