NKG2A、NKG2C受体及其配体HLA-E在子痫前期患者蜕膜中的表达

Expression of NKG2A and NKG2C Receptors and Their Ligand HLA-E in Decidua of Preeclampsia Patients

目的研究杀伤细胞C型凝集素样受体-1(KLC1或NKG2A)和受体-2(KLC2或NKG2C)及其配体人类白细胞抗原(HLA)-E在子痫前期患者蜕膜中的表达水平,探讨其与子痫前期发病的关系。方法收集46例正常足月妊娠孕妇、30例轻度子痫前期和42例重度子痫前期患者的蜕膜标本,采用逆转录-聚合酶链反应法(RTPCR)和免疫组化法检测蜕膜中NKG2A、NKG2CmRNA和受体蛋白的表达,以及HLA-E mRNA的表达。结果HLA-E mRNA的表达水平在子痫前期患者,特别是重度子痫前期患者蜕膜中降低(P<0.05)。重度子痫前期患者NKG2A、NKG2CmRNA和受体蛋白表达水平较正常足月妊娠孕妇增加(P<0.05),重度子痫前期患者NKG2A和NKG2C表达水平之比较正常足月妊娠孕妇低(P<0.05),且重度子痫前期组中NKG2C mRNA和受体蛋白表达水平较NKG2A高(P<0.05)。HLA-E和NKG2A、NKG2C mRNA表达水平均与孕晚期血压、24h尿蛋白等临床及实验室指标相关。结论 HLA-E表达降低和NKG2A、NKG2C不均衡地表达增加可能参与了子痫前期发病机制。

Objective To investigatethe expressions of NKG2A,NKG2Creceptors and their ligand HLA-E in decidua of preeclampsia patients. Methods Decidua tissues were collected from 30 patients with mild preeclampsia,42patients with severe preeclampsia and 46normal pregnancy as contrast.The expressions of NKG2A,NKG2Cprotein were detected by immunohistochemitry and mRNAs of NKG2A,NKG2Cand HLA-E genes were detected by RT-PCR.Results The expression of HLA-E mRNA was significantly decreased in preeclampsia,especially in severe preeclampsia patients（P＆lt;0.05）;The mRNA and protein expression of NKG2A,NKG2Cin severe preeclampsia group were significantly higher than that in normal pregnancy group（P＆lt;0.05）;In the severe preeclampsia group,the ratio of expression level of NKG2Aand NKG2Cwas significantly lower than that of normal group and the mRNA and protein expression ofNKG2Creceptor were both significantly higher than that of NKG2A（P＆lt;0.05）;The mRNA expression level of HLA-E,NKG2Aand NKG2C were closely related with clinical and biochemical indexes,such as blood pressure of late pregnancy and 24-hour proteinuria.Conclusion The decreased expression of HLA-E and the unevenly increase of NKG2Aand NKG2Cmay involve in the pathogenesis of preeclampsia.