卵巢上皮性癌组织eIF-4A蛋白表达临床意义研究

Expression of eIF-4Ain epithelial ovarian carcinoma and its clinical significance

目的:探讨真核细胞翻译起始因子eIF-4A在卵巢上皮性癌组织中的表达及其临床意义。方法:选取青岛大学医学院附属医院妇科2008-01-01-2012-12-31住院病例。应用免疫组织化学方法测定41例卵巢上皮性癌组织、23例交界性卵巢上皮性肿瘤组织、25例良性卵巢上皮性肿瘤组织及30例正常卵巢组织中eIF-4A蛋白的表达情况。结果:eIF-4A蛋白在正常卵巢组织(3.33%)和卵巢交界性肿瘤组织(60.87%)中阳性表达率差异有统计学意义,z=4.607,P<0.001;在卵巢良性肿瘤组织(12.00%)及卵巢交界性肿瘤组织中的阳性表达率差异有统计学意义,z=3.638,P<0.001;在卵巢交界性肿瘤组织和卵巢上皮性癌组织(87.80%)中阳性表达率差异有统计学意义,z=2.399,P=0.016。卵巢良性肿瘤组织和正常卵巢组织中eIF-4A蛋白的表达差异无统计学意义,z=1.239,P=0.215。eIF-4A蛋白在卵巢上皮性癌组织中的阳性表达与FIGO手术病理分期(χ2=7.080,P=0.020)、组织分级(P=0.002)及是否有淋巴结转移(P=0.008)呈明显的相关性;与患者的年龄(χ2=0.035,P=1.00)及卵巢上皮癌的病理类型(χ2=0.093,P=1.00)无明显的相关性。结论:eIF-4A可能参与卵巢上皮性癌的发生、发展并参与淋巴结转移过程,eIF-4A蛋白可能成为治疗卵巢上皮性癌的一个潜在的分子靶点。

OBJECTIVE： To explore the expression and clinical significance of eIF-4A in epithelial ovarian carcinoma tissues. METHODS：The hospitalized cases were selected from 2008-01-01 to 2012-12-31 in Department of Gyrecology,Affiliated Hospital of Medical College of Qingdao University. The expression of eIF-4A was detected by immunohistochemical method of SP in 41 cases of epithelial ovarian cancer,23 cases of the borderline epithelial neoplasia,25 cases of the benign epithelial neoplasia and 30 cases of normal ovarian tissue. RESULTS： The positive rates of eIF4A were 3.33% and 60.87 % respectively and showed significant statistical difference（z= 4. 607, P〈0. 001） in normal ovarian tissues and borderline epithelial neoplasia groups. It had the same results in benign epithelial neoplasia groups and borderline epithelial neoplasia groups,12.00% and 60.87% （z= 3. 638, P〈0. 001）. The expressions of eIF-4A in epithelial ovarian cancer groups were significantly higher than that in borderline epithelial neoplasia groups （z=2. 399, P= 0. 016）. The positive rates of eIF4A in normal ovarian tissues and benign epithelial neoplasia groups were 3.33% and 12.00% respectively and had no significant statistical difference（z= 1. 239, P= 0. 215）. The positive expression of eIF-4A was significantly related to the later FIGO stage（X2 =7. 080,P=0. 020） ,differentiation of tumor（P=0. 002） and lymph node metastasis of epithelial ovarian carcinoma（P=0. 008）, but it had no significant correlation with age（ X2 = 0. 035, P = 1.00） and the his topathological types（X2 = 0. 093, P= 1.00）. CONCLUSIONS： The positive expression of eIF-4A may play a role in the oncogenesis and malignant progression of epithelial ovarian carcinoma. It may be one of useful molecular markers for diagnosis,identifying cancer and predicting malignant progression of epithelial ovarian carcinoma.