乌塌菜种质资源遗传多样性的ISSR分析

ISSR Analysis on Genetic Diversity of Brassica campestris L.var.rosularis Germplasm Resources

利用ISSR技术对48份乌塌菜种质资源进行遗传多样性分析。从60条随机引物中筛选出稳定性强、条带清晰且多态性丰富的9条引物进行PCR扩增，共扩增出103条谱带，平均每个引物扩增出11．4条带，其中多态性带85条，多态性位点百分率为82．68％。不同乌塌菜种质间遗传相似系数变幅为0．59—0．97，说明ISSR标记能够揭示材料间较高的遗传多样性。利用UPGMA聚类分析，ISSR标记能将48份乌塌菜品种完全区分开，48份乌塌菜种质被划分为4个类群，聚类结果与叶片颜色相关，为乌塌菜品种资源的研究利用提供参考。

ISSR markers were used to analyze the genetic diversity of 48 Brassica campestris cultivars. A total of 103 markers were scored from 9 primers, which were screened out from 60 random primers and demonstrated highly reproducible, clear and polymorphic bands. Each primer produced 11.4 bands. Among the obtained markers, 85 bands were polymorphic, accounting for 82.68% of the total. The genetic similarity coefficients among the cultivars ranged from 0.59 to 0.97, reflecting the high genetic diversity of B. campestris germplasms by ISSR markers. Cluster analysis with UPGMA method showed that these 48 germplasms could be divided into four groups, which were closely related to the leaf color of the different cultivars. The results will provide the reference to the study and usage of B. campestris germplasms.