姜黄素对七氟醚吸入后老年大鼠认知功能的影响

Curcumin mitigates the changes of cognitive function by sevoflurane anesthesia in aged rats

目的:观察姜黄素对七氟醚所致老年大鼠学习记忆力改变的影响以及对环氧合酶-2(COX-2)表达的影响。方法:将48只18月龄的SD老年大鼠,采用数字表法随机分为3组。分别为姜黄素组(n=16);七氟醚对照组(n=16);空白对照组(n=16)。采用Morris水迷宫检测大鼠的空间学习记忆功能,以及检测COX-2、β淀粉样蛋白(β-amyloid peptide,Aβ)以及人β-分泌酶(human beta-site APP-cleaving enzyme-1,BACE-1)的表达。结果:(1)Morris水迷宫进行定位航行实验和空间探索实验表明,七氟醚组及姜黄素组的老年SD大鼠学习记忆与空白对照组相比,平均逃避潜伏期延长,穿越平台象限的次数减少(P<0.05),七氟醚组较姜黄素组平均逃避潜伏期及穿越平台象限的次数显著减少(P<0.05);(2)大鼠海马区Aβ的表达:七氟醚组明显高于姜黄素组及空白对照组(P<0.05),而姜黄素组较空白对照组亦有明显差异(P<0.05);(3)七氟醚组、姜黄素组BACE-1的表达明显高于空白对照组(P<0.05);(4)七氟醚组、姜黄素组大鼠海马区COX-2的表达较空白对照组无显著差异(P>0.05)。结论:姜黄素有助于改善高浓度七氟醚所致老年SD大鼠学习记忆力损害,其机制可能与下调BACE-1表达从而抑制高浓度七氟醚诱导Aβ的生成有关,但对COX-2表达无显著影响。

AIM： To investigate the curcumin effect on cognitive function after sevoflurane anesthesia with high concentration and the expres- sion of Cyclooxygenase-2 in aged rats. METH- ODS： Forty-six male Sprague Dawley SD rats aged 18 months weighing 490-590 g were ran- domyly divided into three groups： Blank control group （n=16）, sevoflurane group （n=16）, 3% sevoflurane and curcumin group （curcumine group, n= 16） ; sevoflurane group and curcumine group received anesthesia for 6 h. The cognitive function was tested with Morris Watermaze. Aβ, COX-2 and BACE-1 expressions in the hippocampus were determined with enzymelinked immunosorbent assays and Western-Blot. RESULTS： Compared with the control group, both latency and the number of seeking for hidden-platform has significant difference in sevoflurane and curcumine groups （P 〈0.05）, there were significant differences between the sevoflurane group and curcumine group （P〈0.05）. Compared with blank control group, the expression of Aβ in the hippocampus had significant differences in curcumine group （P〈0.05）, but no significant differences between the blank control and curcumine groups（P〈0.05）. Compared with the blank control group, the expression of BACE-1 in hippocampal was significant increased in sevoflurane and curcumine groups （P〈0.05）. There were no significant differences among the sevoflurane group, curcumine group and blank control group （P 〈 0.05）. CONCLU- SION： Curcumin might mitigate cognitive function impairment induced by sevoflurane with high concentration. Possibly by suppress APP, Aβ generation and caused down-regulation of BACE-1.