北京地区实验动物中嗜肺巴斯德杆菌的表型分析

Phenotypic analysis of Pasteurella pneumotropica in laboratory animals in Beijing area

目的对北京地区实验动物中的嗜肺巴斯德杆菌分离株进行表型分析,提高检测的准确性。方法通过生化鉴定和16S rDNA测序,对306株嗜肺巴斯德杆菌可疑菌株进行鉴定。结合分离株在血琼脂平皿上的菌落特征和生化特性,组成53个特征数据谱,进行表型特征的聚类分析。结果 306株分离株中,BD自动细菌鉴定系统和16S rDNA测序鉴定出嗜肺巴斯德杆菌的阳性率分别为164/306和227/306。227株嗜肺巴斯德杆菌真阳性的表型特征共有140种,Heyl型106种,Jawetz型23种。结论北京地区实验动物中嗜肺巴斯德杆菌主要为Heyl型感染,Jawetz型较少,表型特征多样,分布广泛。

Objective To improve the accuracy of detection through analyzing the phenotypes of P. pneumotropica isolates in laboratory animals in Beijing area. Methods 306 suspicious P. pneumotropica strains were identified by biochemical identification and 16S rDNA sequencing. Then,to obtain the phylogenetic relationships combined with colony characteristics on blood agar plates and biochemical characteristics of 53 biotypes. Results BD Phoenix 100 automated bacterial identification system and 16S rDNA sequencing identified P. pneumotropica positive rate of 306 isolates were 164 /306 and 227 /306,respectively. There were 140 phenotypes in 227 true-positive strains,of which 106 were biotype Heyl and 23 were biotype Jawetz. Conclusions In the samples of laboratory animals in Beijing area,P. pneumotropica infection mainly are of biotype Heyl,and less is of biotype Jawetz. The phenotypes are diverse and widely distributed.