Pg-lps对大鼠血管平滑肌细胞增殖和ALP活性的影响

Proliferation and ALP ase activity of rat vascular smooth muscle cells in response to Porphyromonas gingivalis lipopolysaccharide in vitro

目的：观察牙龈卟啉单胞菌脂多糖（Pg－lps）对体外培养大鼠血管平滑肌细胞（vascular smooth muscle cells，VSMCs）增殖和碱性磷酸酶（ALP）活性的影响。方法：采用组织块贴壁法培养大鼠VSMCs，传代培养并鉴定。Pg－lps （1～10000 ng／mL）干预VSMCs 0．5～4 d，采用CCK－8法和碱性磷酸酶试剂盒测定其增殖和ALP活性。结果：1 d后（1－10000 ng／mL）Pg－lps均显著促进VSMCs增殖，并随时间的延长，促进作用越明显；而（1 ng／mL、1μg／mL）Pg－lps在0．5～4 d均显著促进VSMCs的ALP活性，随着时间的延长促进作用更明显，且4 d时1μg／mL较1 ng／mL对ALP活性促进作用更明显。结论：Pg－lps能促进VSMCs的异常增殖；还可能通过促进VSMCs的ALP活性而影响其钙化过程，进而提示Pg－lps对心血管疾病的发生发展可能有重要作用。

Objective：To investigate the effect of Porphyromonas gingivalis lipopolysaccharide（Pg-lps）on proliferation and ALPase activity of rat vascular smooth muscle cells （VSMCs）in vitro. Method：The primary culture of VSMCs were cul-tured in vitro with the tissue adherent method. The cells were purified by differential adherent and natural growth purification and immunocytochemistry staining were used to identify the immunological characteristics.The proliferation and ALPase ac-tivity of cultured VSMCs by using CCK-8 method and alkaline phosphatase activity test. Result：After 1 day（1~10000 ng/mL） Pg-lps all promoted proliferation of VSMCs significantly,and as the extension of time promoting effect was more appar-ent;while（1 ng/mL,1 μg/mL） Pg-lps significantly promoted the ALP ase activity of VSMCs in（0.5~4 days）,and as the extension of time promoting effect was more apparent;1μg/mL Pg-lps promoted ALPase activity stronger than the effect of 1 ng/mL Pg-lps. Conclusion：Pg-lps can promote abnormal proliferation of VSMCs;it possibly affects calcification process of VSMCs by promoting its ALPase activity;thus that prompts Pg-lps may play an important role for the development of car-diovascular disease.