骨髓间充质干细胞旁分泌对急性心肌梗死心肌的保护作用（英文）

Bone marrow mesenchymal stem cells protect myocardial function in acute myocardial infarction through a paracrine mechanism

背景:成人心肌细胞缺乏再生能力,使用细胞疗法再生和修复心肌,可能提高心肌缺血性损伤后功能。目的:探索骨髓间充质干细胞治疗急性心肌梗死中可能的作用机制。方法:密度梯度离心法从SD大鼠中分离培养骨髓间充质干细胞。20只大鼠以开胸冠状动脉结扎法制备急性心肌梗死模型后,随机等分为模型组和骨髓间充质干细胞组,骨髓间充质干细胞组大鼠于冠状动脉结扎后通过尾静脉注射骨髓间充质干细胞。结果与结论:造模6个月后,与模型组相比,骨髓间充质干细胞组大鼠心脏功能明显改善,心肌组织血管密度增加,细胞凋亡数量减少,心脏组织中炎症因子血管内皮生长因子、von Willebrand因子、肿瘤生长因子3β和白细胞介素1βmRNA表达明显增加。提示骨髓间充质干细胞通过调节心脏炎症因子与血管因子的分泌起到保护急性心肌梗死后心肌细胞的作用。

BACKGROUND：Adult cardiomyocytes show no regenerative ability, and celltherapy for myocardial regeneration and repair may improve myocardial ischemic injury function. OBJECTIVE：To confirm the effect and reveal the mechanism of bone marrow mesenchymal stem cells （BMSCs） on acute myocardial infarction （AMI）. METHODS：BMSCs were isolated, cultured from bone marrow of Sprague-Dawley rats using density gradient centrifugation. AMI models were produced in 20 rats by ligating the left anterior descending （LAD） coronary artery, and randomly divided into model group and BMSCs group. In the BMSCs group, cells were subsequently injected with a sterile microinjection via the tail vein. RESULTS AND CONCLUSION：Six months postoperatively, the cardiac function was improved, the vessel density was increased, the percentage of apoptotic cells was decreased in the BMSCs group than that in the model group;the expression levels of inflammatory factors, including vascular endothelial growth factor, von Wil ebrand factor, transforming growth factor 3β, and interleukin-1βmRNA were significantly improved in the BMSCs group than that in the model group. These results showed that BMSCs can protect the myocardium from AMI by regulating the secretion of inflammatory cytokines and angiogenic factors.