高原习服进程中淋巴细胞线粒体生物合成和自噬的变化规律

Changes of mitochondrial biogenesis and mitophagy in leukocyte during acclimatization to high-altitude

【目的】观察世居平原男性青年高原习服各时程淋巴细胞线粒体生物合成和自噬的动态变化规律。【方法】27例世居平原武警新兵急进高原，分别在移居高原3、7、90d检测淋巴细胞线粒体DNA（mtDNA）中8-oxodG含量、mtDNA拷贝数、PGC-1α、Tfam、Bnip3和Beclin-1蛋白表达。【结果】与平原阶段比较，移居高原3d和7d，mtDNA拷贝数、8-oxodG含量、PGC-1α、Tram、BniP3和Beclin-1蛋白表达显著升高（P〈0．05．0．01）；移居高原90d，mtDNA拷贝数、PGC-1α和Tfam蛋白表达显著降低（P〈0．05），Bnip3和Beclin-1蛋白表达显著升高（P〈O．01）。与移居高原7d比较，移居高原90d，mtDNA拷贝数、8-oxodG含量、PGC-1α和Tfam蛋白表达显著降低（P〈O．05～0．01），Bnip3和Beclin-1蛋白表达显著升高（P〈0．05）。【结论】高原低氧习服初期，淋巴细胞线粒体重构主要依赖于线粒体生物合成增加以提高线粒体数量；而在高原习服后期主要依赖于线粒体白噬增加以提高线粒体质量。

[Objective] This study is aimed to explore the change of mitochondrial biogenesis and mitophagy in leukocyte during acclimatization to high-altitude hypoxia. [ Methods ] Twenty-seven lowlanders quickly entered plateau. Leukocyte mitochondrial DNA （mtDNA） copies, 8-oxodG content in mtDNA, PGC-1α, Tfam, Bnip3 and Beclin-1 protein expression were respectively measured in plain, in plateau at 3,7,90 days after entry. [Results]As compared with plain group, mtDNA copies, 8-oxodG content, PGC-1α, Tfam, Bnip3 and Beclin- 1 level significantly increased at 3 days and 7 days （P 〈 0.05）, mtl）NA copies, PGC- 1α and Tfam level markedly decreased at 90 days （P 〈 0.05）, Bnip3 and Beclin-1 level significantly increased at 90 days （P 〈 0.01）. As compared with 7 d group, mtDNA copies, 8- oxodG content, PGC- 1α and Tfam level significantly decreased （P 〈 0.05）, Bnip3 and Beclin- 1 level markedly increased in 90 days （P 〈 0.05）. [Conclusion ]In the early phase of acclimatization to high-altitude, leukocyte mitochondrial remodeling mainly depended on increased mitochondrial biogenesis and content, while in the latter phase of acclimatization, leukocyte mitochondrial remodeling mainly depended on the elevation of mitophagy and mitochondrial quality.