靶向抗肿瘤GnRH-PE39KDEL融合蛋白表达培养基的优化

Medium Optimization for Expressing GnRH-PE39KDEL Fusion Protein in Engineered Escherichia coli

通过筛选培养基、单因子筛选试验及正交试验进行摇瓶发酵,确定适合该菌株表达融合蛋白的最佳培养基配方。采用该优化培养基可使抗肿瘤GnRH-PE39KDEL融合蛋白表达量占总蛋白的34.5%,占可溶蛋白的40%。

Fermentation medium of the engineered Escherichia coli/GnRH-PE39KDEL for expressing the fusion protein was optimized by selecting medium,single factor and orthogonal test.The yield of the fusion protein in optimized fermentation medium reached to 34.5％ of the total protein and 40％ of soluble protein.