三七总皂苷预处理对急性内脏痛大鼠星形胶质细胞的影响

The Effect of TSPN Preconditioning on Astrocytes in Rats of Visceral Pain

目的:探讨三七总皂苷预处理对急性内脏痛大鼠的影响,初步阐述三七总皂苷对急性内脏痛的影响机制。方法:成年雌性SD大鼠54只随机分为正常组(n=6),生理盐水预处理组(n=24),三七总皂苷预处理组(n=24)。正常组常规条件饲养,不做干预及建立急性内脏痛模型;生理盐水预处理组和三七总皂苷预处理组大鼠分别预先腹腔注射生理盐水(2.86 ml/kg)或7 mg/ml的三七总皂苷(2.86 ml/kg),每12 h一次,连续7 d,第8天腹腔注射1%乙酸(10 mg/kg),建立急性内脏痛模型,立即观测SD大鼠扭体反应。按(30、60、90、180 min)不同存活时间处死动物,免疫组化法观测脊髓背角GFAP的表达变化。结果:VPI评分显示,三七总皂苷能显著下调内脏痛模型VPI评分,减轻疼痛。免疫组化显示在相同时间点,三七总皂苷预处理组大鼠GFAP表达弱于生理盐水预处理组,尤其在30、60、90 min存活组。结论:对SD大鼠急性内脏痛模型预先腹腔注射三七总皂苷可以抑制脊髓胶质细胞激活,从而减轻急性内脏痛。

Objective： To investigate the effect of TSPN preconditioning on acute visceral pain rats and expound correlated mechanism. Methods： 54 female adult SD rats were randomly divided into normal group （n=6）, NS preconditioning group （n=24）, TSPN preconditioning group （n=24）. The normal group rats were fed routinely, proceed without drug intervention and intraperitoneal injection of acetic acid; The NS group and the TSPN group rats received an intraperitoneal injection of 1% 10mg/kg acetic acid （10 ml/kg） after intraperitoneal preadministration of normal saline（2.86 ml/kg） or 7 mg/ml TSPN（2.86 ml/kg） twice a day for 7d. On the eighth day, the rats of preconditioning groups were sacrificed at different intervals （30, 60, 90, 180 rain）, randomly. The writhing test was measured immedi- ately after the acetic acid injected and the expression of GFAP in spinal cord was compared. Results： VPI scores showed that compared with the NS preconditioning, TSPN preconditioning can significantly decrease VPI scores of the acute visceral pain rats, immunohisto- chemistry showed that the GFAP expression of the TSPN preconditioning group rats was in the same trend with the NS preconditioning group rats, but was weaker than the NS preconditioning group at the same time point, in particular, at 30, 60, 90 min survival group. Con- elusion： Intraperitoneal preadministration of TSPN is able to inhibit the activation of astrocytes in the Superficial Laminae of spinal dorsal horn of SD rats, attenuating acute visceral pain.