夹心ELISA法测定正常人血浆sDC-SIGN浓度及其意义初步探讨

Preliminary study of soluble DC-SIGN concentration by ELISA in healthy volunteers

目的测定正常人血浆sDC-SIGN浓度。方法对本室建立的夹心ELISA法检测sDC-SIGN体系进行灵敏度和重复性评估,并采用该体系对352例健康志愿者外周血进行sDC-SIGN浓度测定。结果本室所建立的检测体系灵敏度可达6 ng/ml,批内变异10.4%,批间变异13.8%;352例志愿者检出sDC-SIGN的比例约58%,中位数1.18 ng/ml,百分之75分位数11.94 ng/ml。结论本室建立的体系可初步用于人血浆sDC-SIGN浓度测定;正常人血浆sDC-SIGN浓度较低,总体呈正偏态分布。

Objective To detect the concentrations of soluble DC-SIGN in normal human plasma. Methods The sensitivity and repeatability experiments were tested respectively for the specific sDC-SIGN ELISA which was developed in our laboratory, and then 352 serum samples from healthy volunteers wered detected for sDC-SIGN concentration by the ELISA. Results The sensitivity of ELISA developed in our laboratory was up to 6 ng/ml and the variation of group and inter batch were 10.4% and 13.8%, respectively. About 58% healthy donors contained detectable amounts of sDC-SIGN ranging from 0 to 187 ng/ml with a median value of 1.18 ng/ml. Conclusions The specific sDC-SIGN ELISA was developed in our laboratory could detect serum sDC-SIGN, and the serum sDC-SIGN concentrations are low in healthy volunteers, which is positive skew distribution.