茵陈蒿汤主要成分的HPLC分析

Qualitative analysis of the main components of YCHT

目的 :通过高效液相色谱法(high performance liquid chromatography,HPLC)对茵陈蒿汤(Yinchenhao decoction,YCHT)中主要成分的进行定性。方法 :采用welch-materials XB-C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈(A)-0.1%磷酸(B)梯度洗脱,流速1.0 mL/min,柱温30℃,分别对8个对照品及YCHT供试品在254、280及440 nm 3个波长下的色谱图进行比较。结果:YCHT中8个有效成分没食子酸、绿原酸、栀子苷及芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚在254 nm处均有良好吸收;没食子酸、绿原酸在280 nm处亦有吸收,440 nm处无吸收;栀子苷在280 nm和440 nm无吸收;5种蒽醌类成分在280 nm及440 nm处均有良好吸收。结论:采用已确定的色谱条件,对YCHT 254、280及440 nm 3个波长的色谱图的比较,可较全面分析其主要成分。

Objective： To investigate the qualitative analysis of the main components of Yinchenhao decoction（YCHT） by high performance liquid chromatography（HPLC）. Methods： The method was established on a Welch-materials XB-C18 column （250 mm ×4.6 mm,5 μm） with mobile phase in gradient elution composed of acetonitrile（A）-0.1%phosphoric acid （B） flowed at 1 mL·min-1. The column temperature was 35 ℃ to test 8 reference substance and the test materials of YCHT on 254 nm, 280 nm, 440 nm wavelengths and compare the chromatograms controls. Results： Eight components in YCHD havebeen iden-tified as gallic acid, chlorogenic acid, aloe Geniposide emodin, rhein, emodin, chrysophanol and physcion were absorbed well at 254 nm. Gallic acid and chlorogenic acid were also absorbed at 280 nm and they were not absorbed at 440 nm. At 280 nm and 440 nm there is no absorption of geniposide. At the 280 nm and 440 nm these five anthraquinones have good ab-sorption. Conclusion： HPLC which chromatographic conditions have been identified at 254 nm, 280 nm, 440 nm wave-lengths, can be used to analyze the major components of YCHT.