幼猪开胸深低温体外循环模型的建立研究

The establishment of the animal model of open chest deep hypothermia cardiopulmonary bypass in the piglets

目的:建立幼猪深低温低流量、深低温停循环体外循环模型,为研究深低温体外循环对自身脏器损伤的研究提供动物模型。方法:16只健康幼猪,平均年龄(24.1±2.7)d,体质量(6.5±0.5)kg,采用开胸体外循环转流降温。16只幼猪随机分为两组,即A组:深低温低流量60 min;B组:深低温停循环60 min,术中进行心电图监测、动脉混合静脉血气分析和血流动力学观察。结果:16只幼猪均成功建立体外循环模型,体外循环后幼猪心肺功能均顺利恢复,血气指标分析均在正常范围,血流动力学在正常范围。结论:开胸成功建立幼猪深低温体外循环模型,为研究深低温体外循环自身脏器损伤提供了动物模型。

Objective：To establish the animal model of open chest deep hypothermia low flow （DHLF） and deep hypothermic circulatory arrest（DHCA） cardiopulmonary bypass （CPB） in the Piglets. And to provide theoretical basis for the researches of important organ injury. Methods：16 healthy piglets, with an average age of （24.1±2.7） d, （6.5±0.5） kg by weight, used open chest cardiopulmonary bypass. 16 piglets were randomly divided into two groups , i.e. A group： deep hypothermic circulatory arrest （DHCA） for 60 minutes;B group：deep hypothermia low flow（DHLF） for 60 minutes, ECG monitoring and mixed venous blood gas analysis of arterial and invasive blood pressure were measured. Results： establish successfully 16 piglets model of cardiopulmonary bypass, lung function after CPB were successfully restored, arterial blood gas analysis were in the normal range, blood pressure was maintained at more than 50 mmHg. Conclusion：The animal model of open chest deep hypothermia CPB can be established successfully in piglets with excellent survive. This model was a stable platform for the study of the pathophysiology of CPB and post-CPB brain dysfunctions.