摘要
体外培养人脐静脉内皮细胞,乳酸脱氢酶(LDH)试剂盒评价王不留行黄酮苷孵育24 h后的细胞毒性。先给予13.76、6.88、3.44μmol/L的王不留行黄酮苷和维生素C阳性对照组(浓度为100μmol/L)预孵育12 h后,再分别以H2O2和高糖诱导内皮细胞损伤。SRB法测定细胞活力,试剂盒检测乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)含量。与模型组相比,王不留行黄酮苷13.76、6.88、3.44μmol/L组均可改善H2O2和高糖损伤模型的细胞活力,尤以13.76μmol/L作用最为显著(P<0.01);并且王不留行黄酮苷13.76μmol/L显著降低H2O2和高糖损伤组培养液中LDH和MDA释放量,增强细胞内SOD活性。
The cytotoxicity of vaecarin on human EA · hy926 endothelial ceils was investigated by measuring the release of lactate dehydrogenase （LDH） in extraceUu]ar fluid. The model of oxidative injury was induced by hydrogen peroxide （ H2 02 ） and the model of high glucose injury was induced by high glucose after treatment of these cells with different doses （13.76,6.88,3.44 μmol/L） of vacearin and 100μmol/L of vitamin C. Then the cell viability was measured by SRB method. The release of LDH, the level of malondialdehyde （MDA） and the activity of superoxide dismutase （SOD） in EA · hy926 were determined according to the manufacturer＇s instructions. Hence,the protective effect of vaccarin a- gainst H202and high glucose induced endothelial cellular damage was confirmed. As shown,the superior effect of vaccar- in was observed at 13.76μmoL/L, which significantly reduced the level of MDA and LDH leakage, and enhanced production of SOD.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第7期1009-1013,共5页
Natural Product Research and Development
