海带多糖对糖尿病小鼠降钙素受体样受体表达的影响

Effect of Laminaria japonica Polysaccharide on the Expression of Calcitonin Receptor-like Receptor in Diabetes Mellitus Model Mice

探讨海带多糖对实验性2型糖尿病小鼠降钙素受体样受体(CrlR)表达的影响及其降糖作用的研究。健康雄性昆明小鼠40只,高脂饲料喂养并腹腔注射四氧嘧啶建立2型糖尿病模型,海带多糖灌胃干预治疗。自动血糖仪检测小鼠空腹血糖(FBG)水平,免疫组织化学和Western blot检测脑干、肝脏和胰腺组织中CrlR蛋白表达,RT-PCR检测CrlR mRNA表达。经海带多糖治疗后,动物血清空腹FBG水平较模型组显著降低(P<0.05);肝脏和胰岛细胞CrlR mRNA及其蛋白表达水平显著升高(P<0.05)。实验表明海带多糖可能通过提高肝脏和胰岛细胞CrlR表达,减轻胰岛素抵抗,发挥降糖作用。

To investigate the effect of Laminariajaponica polysaccharide （UPS） on the expression of calcitonin receptor- like receptor ＂（CrlR） and the hypoglycemic effect on type 2 diabetes mellitus mice. Type 2 diabetes mellitus mouse model was established by feecling high fatty forage and injecting alloxan intraperitoneally in 40 healthy male mice and treated with LIPS orally. The level of fasting blood glucose （FBG） was detected by automatic blood glucose device. The expression of CrlR was determined by immunohistochemisty and Western blot, and the expression of CrIR mRNA was detected by RT-PCR. After treated with L. japonica, the serum level of FBG were decreased significantly compared with that of model group （P 〈 0.05 ）. In the liver and pancreas, the expression levels of CrlR mRNA and CrlR protein were significantly higher than those in model group （P 〈 0.05）. This experiment showed that LJPS played a hypoglycemic effect by promoting the expression of CrlR in liver and pancreatic tissue to lessen insulin resistance （IR）.