胃癌患者CD4^+CD29^+T细胞表达及其临床意义

Expression and significance of CD4+CD29+T cells for patients with gastric carcinoma

目的探讨CD4+CD29+辅助性T细胞在胃癌患者中的表达及其临床意义。方法收集Ⅰ~Ⅳ期胃癌患者共64例、慢性胃炎患者及健康对照组各60例作为观察对象。采用流式细胞术检测外周血CD4+CD29+T细胞及其细胞因子γ干扰素(IFN-γ)及白介素4(IL-4)水平;采用ELISA法检测三组的细胞间黏附分子-1(ICAM-1)、转化生长因子β1(TGF-β1)及癌胚抗原(CEA)的含量;分析以上六者的相关性。结果胃癌患者CD4+CD29+T细胞及IL-4水平显著高于慢性胃炎及健康对照组(P<0.05),而IFN-γ明显低于另外两组(P<0.05),但慢性胃炎与健康对照组上述三项指标无统计学差异。Ⅰ~Ⅳ期胃癌患者CD4+CD29+T细胞及IL-4呈递增趋势,而IFN-γ呈递减趋势,均表现为Ⅲ与Ⅰ期之间、Ⅳ与Ⅲ期之间均存在显著差异(P<0.05)。胃癌患者ICAM-1、TGF-β1及CEA显著高于慢性胃炎及健康对照组(P<0.05),但慢性胃炎与健康对照组上述三项指标无统计学差异。相关性分析显示CD4+CD29+T细胞及IL-4两者与ICAM-1、TGF-β1及CEA三者当中任意一者均呈正相关性,而IFN-γ与ICAM-1、TGF-β1及CEA三者当中任意一者均呈负相关性(P<0.05)。结论 CD4+CD29+T细胞及IL-4表达增加、IFN-γ表达减少是胃癌患者的一个重要免疫学特征,在早晚期胃癌呈显著差异性表达,且三者与胃癌的血管表达相关因子密切相关。

Objective To explore the expression and significance of CD4^＋CD29^＋T cells for patients with gastric carcinoma. Methods Sixty-four patients with gastric cancer, including I -IV stage, were enrolled, as well 60 chronic gastritis patients and healthy subjects. The peripheral blood CD4^＋CD29^＋T cells and cytokines T-interferon （IFN-γ） and interleukin 4 （IL-4） were tested using flow cytometry. The levels of intercellular adhesion molecule 1 （ICAM- 1）, transforming growth factor beta 1 （TGF-beta 1） and carcinoembryonic antigen （CEA） were also tested. The correlation between the above-mentioned 6 indexes was analyzed via Pearson test. Results The percentage of CD4^＋CD29^＋ T cells and mean fluorescence intensity of IL-4 of the gastric cancer patients were higher than those in the chronic gastritis and healthy subjects （P〈0.05）; whereas the mean fluorescence intensity of IFN-γ were lower than the other two groups for the gastric cancer patients. CD4^＋CD29^＋T cells and IL-4 increased and but IFN-γ decreased for the I to IV stage gastric cancer patients, and the levels of ICAM-1, TGF-131and CEA also increased when compared with the other two groups （P〈0.05）. Pearson analysis showed that CD4^＋CD29^＋F cells and IL-4 positively correlate with CAM-l, TGF-β1 and CEA; but IFN-γ negatively correlate with CAM-1, TGF-β1 and CEA （P〈0.05）. Conclusion Increase of CD4^＋CD29^＋T cells and IL-4, as well the decrease of IFN-γ were the important immunological features for gastric cancer patients. CD4^＋CD29^＋T cells, IL-4 and IFN-γ were differently expressed in early and end stages for patients with gastric carcinoma significantly, and they correlated with the factors which are closely related the tumor angiogenesis.