穿心莲内酯抗子宫内膜异位症的药效学评价

Effect of andrographolide in experimental endometriosis models

目的:利用大鼠、小鼠子宫内膜异位症模型,对穿心莲内酯改善子宫内膜异位症药效进行初步评价。方法:采用口服他莫昔酚建立小鼠子宫内膜异位症模型,利用热板实验筛选成模小鼠。给药4周,末次实验后处死动物,取双侧子宫进行病理组织学检查。采用自体移植法建立大鼠子宫内膜异位症模型,术后2 d开始给药,连续4周,末次给药后测定各组大鼠异位子宫内膜组织体积大小,进行病理组织学观察及血清中雌二醇和孕酮水平检测。结果:穿心莲内酯及达那唑治疗4周后,小鼠痛阈值较模型组均明显提高(P<0.05或0.01);腺肌症发生率及内膜浸润趋势有减少趋势。穿心莲内酯高剂(30 mg·kg-1)可明显缩小异位子宫体积(P<0.01)。结论:穿心莲内酯具有一定治疗子宫内膜异位症的作用,并对其疼痛症状有一定的改善。

Objective：To determine the effect of andrographolide on sexual hormone and other changes in rat and mouse models of endometriosis.Methods：In this study,adenomyosis was induced by tamoxifen in ICR neonatal mice,and the mice were treated with andrographolide for 4 weeks.The response to thermal stimuli in hotplate tests was evaluated after the last andrographolide administration.Endometriosis was induced in rats by surgery of self-endometrium transplantation.Rats were then treated with andrographolide for 4 weeks.The levels of E2 and P were detected by radioimmunoassay.Results：In mice with experimental endometriosis,andrographolide and danazol efficaciously improved generalized hyperalgesia（P〈0.05 or 0.01） 4 weeks after treatment.These drugs showed a tendency to reduce the myometrial infiltration;and mildly,but not significantly,decreased uterus and uterine coefficients compared with the model control.In rat endometriosis model,andrographolide at 30 mg·kg^- 1 significantly reduced ectopic uterine volume（P〈0.01）,but estrogen and progesterone levels had no significant changes compared with model control.Conclusion：Andrographolide has a partial effect on endometriosis,especially relieving pain.