商陆皂苷甲对BXSB小鼠肾组织PCNA、Caspase-3、Fas和FasL表达影响

Effect of Esculentoside A on Apoptosis and Expression of PCNA,Caspase-3,Fas and FasL in Renal Tissue of BXSB Mice

[目的]观察商陆皂苷甲(esculentosideA,EsA)对BXSB小鼠肾组织细胞增殖核抗原(PCNA)、半胱氨酸天冬氨酸酶3(Caspase-3)、凋亡诱导因子(Fas)和凋亡诱导因子配体(FasL)蛋白表达影响。[方法]将24只18周龄雄性BXSB小鼠随机分为模型组、地塞米松组和商陆皂苷甲(EsA)组,8只/组。腹腔注射干预:模型组RPMI1640培养液0.2mL/d;地塞米松组,地塞米松溶液1mg/kg·d;商陆皂苷甲组,EsA溶液20mg/kg·d;每只小鼠1次/d,连续4周,处死小鼠取肾组织标本检测(终点为小鼠22周龄)。干预始点及结束前,留取尿标本检测尿蛋白和尿肌酐浓度。[结果]与模型组相比,商陆皂苷甲组和地塞米松组尿蛋白/肌酐比值下降,肾脏病理变化有不同程度改善,肾组织中PCNA表达减弱,肾组织Caspase-3、Fas和FasL表达增强(P<0.05)。[结论]实验剂量EsA和地塞米松能够降低BXSB小鼠尿蛋白,改善肾脏病理变化;EsA和地塞米松减轻BXSB小鼠肾损害可能机制是通过抑制BXSB小鼠肾组织细胞增殖和促进肾组织细胞凋亡实现。

[ Objective ] To explain the mechanism of EsA effects LN and effects on the expression of PCNA, Caspase-3, Fas and FasL in renal tissue of BXSB mice. [ Methods ] 24 male BXSB mice at the age of 18 weeks were randomly divided into three groups ：model group, EsA-treated group and Dexamethasonetreated group. Each group were treated, all mice were obtained urine randomly, and were killed to obtain renal tissue after 4 weeks.For the index test. [ Results ] The level of urine protein/urine creatinine was decreased in EsA-treated group and Dexamethasone-treated group （ P〈0.05 ） ; After using drug, the renal pathological change of BXSB mice moderates significantly;The expression of PCNA mRNA in renal tissue of model group was significantly higher than it of EsA-treated group and Dexamethasone-treated group （P〈0.05） ;As for the expression of Caspase-3 mRNA, Fas and FasL in renal tissue of model group was lower than them of EsA- treated group and Dexamethasone-treated group, and there has significance in three groups （ P〈0.05 ） . [ Conclusion ] Experimental doses of EsA and Dexamethasone can degrade urine protein concentration and improve renal pathology, so they can reduce renal damage of BXSB mice;EsA and Dexamethasone can reduce renal damage by the potential meshanism of inhibiting renal cell proliferation and improving renal cell apoptosis of BXSB mice.