鱼藤素对小细胞肺癌的体外抗肿瘤效果评价及相关机制分析

The antitumor activities and mechanisms of deguelin in small cell lung cancer in vitro

目的:研究鱼藤素对小细胞肺癌体外抗肿瘤效果。方法:0、5、10、20、50μmol/L鱼藤素作用于NCI-H446细胞24 h,细胞计数试剂盒8(cell counting kit-8,CCK8)分析鱼藤素对细胞活力的影响。据CCK8结果,以0、5、10、20μmol/L鱼藤素作用于NCI-H446细胞,EdU掺入法检测细胞增殖,DAPI染色及流式细胞术测定细胞凋亡。据流式结果,20μmol/L鱼藤素作用NCIH446细胞0、0.5、2.0、4.0 h,蛋白质印迹法检测细胞内凋亡相关蛋白的表达。结果:鱼藤素呈剂量依赖性地抑制小细胞肺癌NCIH446细胞增殖、诱导其凋亡,作用不同时间后可上调促凋亡蛋白Bax、下调抗凋亡蛋白Bcl-2水平。结论:鱼藤素体外抗小细胞肺癌效应可能通过上调促凋亡蛋白Bax、下调抗凋亡蛋白Bcl-2表达,诱导细胞凋亡实现。

Objective：To investigate the antitumor activity of deguelin in small cell lung cancer and the possible underlying mechanisms.Methods：NCI-H446 cells were treated with deguelin at 0,5,10,20 and 50 μmol/L for 24 hours.The effect of deguelin on cell activity of NCI-H446 cells was evaluated by CCK8.EdU assay and DAPI staining were performed to assess cellular proliferation and apoptosis,respectively.According to flow cytometry analysis,20 μmol/L deguelin were given NCI-H446 cells for0,0.5,2.0 and 4.0 hours,respectively.Western blot was applied to detect the levels of proteins involved in apoptosis.Results：Deguelin significantly inhibited proliferation and induced apoptosis of NCI-H446 in a dose-dependent manner.After treating with deguelin for different time lengths,pro-apoptotic protein Bax level was increased and anti-apoptotic protein Bcl-2 level was decreased.Conclusion：Deguelin suppresses small cell lung cancer in vitro via promoting its apoptosis probably by up-regulating pro-apoptotic protein Bax and down-regulating anti-apoptotic protein Bcl-2.