摘要
目的:观察肝细胞生长因子(HGF)对人胆管细胞型肝癌细胞HCCC-9810增殖的影响,并探讨其分子机制。方法 :HGF处理HCCC-9810细胞后,用MTT及EdU法检测癌细胞的增殖;siRNA干扰技术抑制HCCC-9810细胞内源性Akt的表达,Western blot检测癌细胞Cyclin D1蛋白表达和Akt磷酸化的变化。结果:50和100 ng/ml HGF显著抑制肝癌细胞HCCC-9810的增殖。Western blot结果显示,HGF处理后的肝癌细胞Akt磷酸化和Cyclin D1表达量明显下调。siRNA干扰Akt的表达,阻断了HGF诱导的Cyclin D1表达量的下调及细胞增殖抑制。结论:HGF通过下调Akt磷酸化和Cyclin D1表达量抑制肝癌细胞HCCC-9810的增殖。
Objective:To identify the novel function of hepatocyte growth factor(HGF) in liver carcinoma cell HCCC-9810proliferation and the underlying molecular mechanism. Methods:The proliferation of HCCC-9810 treated with HGF was detected by MTT assay and EdU assay. The expression of Akt in HCCC-9810 cells was inhibited by siRNA. Western blot was used to detect the expression of cyclin D1 and the phosphorylation level of Akt. Results:Both 50 ng / ml and 100 ng / ml HGF significantly inhibited the proliferation of HCCC-9810. Western blotting assay showed that HGF downregulated the expression of cyclin D1 and the phosphorylation level of Akt in HCCC-9810 cells. Conclusion:These results suggest that HGF inhibited the proliferation of HCCC-9810 by suppressing the expression of cyclin D1 and the phosphorylation level of Akt. SiRNA interfered the expression of Akt,and blocked HGF-induced downregulation of cyclin D1 expression and inhibition of cell proliferation.
出处
《南京医科大学学报(自然科学版)》
CAS
CSCD
北大核心
2014年第7期870-874,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省苏北人民医院院级科研基金项目(yzucms201318)