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转录因子Yin Yang1(YY1)在食管癌中的表达及其对食管癌细胞功能的影响 被引量：3

The expression of transcription factor Yin Yang 1(YY1) and its function in esophageal squamous cell carcinoma
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摘要 目的:探讨转录因子Yin Yang 1(YY1)在正常食管组织和食管鳞癌组织中的表达及其在食管癌发生发展中的作用。方法:免疫组化染色检测15例正常食管组织,39例食管癌旁组织和88例食管癌组织样品中YY1蛋白表达水平;噻唑蓝(MTT)和克隆实验检测食管鳞癌细胞(TE-1细胞)增殖和生长变化;蛋白质印迹(Western blot)技术检测转染后转录因子YY1和p21表达水平;细胞划痕实验和Transwell实验检测TE-1细胞迁移、侵袭能力的变化。结果:与正常食管组织和食管癌旁组织相比,YY1蛋白在食管鳞癌组织样品中的表达显著提高。此外,转染了YY1过表达载体后明显抑制TE-1细胞生长,而干扰YY1表达则可促进细胞的生长;同时伴随着YY1靶基因p21(也称p21WAF1/Cip1)的变化。TE-1细胞转染YY1过表达载体后,细胞的侵袭、迁移能力增强。结论:食管鳞癌组织中YY1表达显著上调,YY1可调节细胞的生长和转移侵袭能力。因此,YY1有望成为食管癌诊断和发展的分子标志物,也有可能是食管癌基因治疗的一个潜在靶点。 Yin Yang-1（YY1） is a member of the zinc finger factor GLI-Krüppel family. Objective：To investigate the gene expression and the function of YY1 in normal esophageal tissues and esophageal squamous carcinoma. Methods：The protein expression of YY1 in 88 esophageal squamous cell carcinomas,39 tumor adjacent tissues and 15 normal esophageal specimens was determined by immunohistochemistry（IHC）. Proliferation and growth changes of esophageal squamous carcinoma cells were measured by MTT and colony formation. YY1 and p21 protein expressions were determined by Western blotting assays. TE-1 cell invasion and migration were determined by cell scratch experiment and Transwell invasive assays. Results：Compared to normal esophageal and tumor adjacent tissues,the expression of YY1 was significantly higher in esophageal squamous cell carcinomas.Furthermore,TE-1 cells overexpressing of YY1 significantly suppressed cell growth and downregulation of YY1 promoted cell growth.Meanwhile,a change of YY1 target gene p21（p21WAF1 / Cip1） was found. TE-1 cells overexpressing YY1 promoted cell invasion and migration. Conclusion：The expression of YY1 was significantly increased and regulated the growth,invasion and migration ability of esophageal squamous cells. Therefore,YY1 is expected to be a molecular marker in the diagnosis and development of esophageal squamous cell carcinoma,and also a potential target for gene therapy of esophagus cancer.
作者 葛欣 谷庆 唐益庭 朱巍 杨红英 张舒羽 曹建平
机构地区 苏州大学医学部放射医学与防护学院
出处 《南京医科大学学报（自然科学版）》 CAS CSCD 北大核心 2014年第7期930-936,共7页 Journal of Nanjing Medical University(Natural Sciences)
基金 国家自然科学基金(81172597 81102078) 江苏省高校重大科研项目(11KJA310001)
关键词 食管鳞癌 YY1 P21 esophageal squamous cell carcinoma（ESCC） YY1 p21
分类号 R735.1 [医药卫生—肿瘤]
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参考文献25

  • 1Park K,Atchison ML.Isolation of a candidate repressor/activator,NF-E1(YY-1,delta),that binds to the im-munoglobulin kappa 3' enhancer and the immunoglobulin heavy-chain mu E1 site [J].Proc Natl Acad Sci U S A,1991,88(21):9804-9808.
  • 2Shi Y,Seto E,Chang LS,et al.Transcriptional repression by YY1,a human GLI-Kruppel-related protein,and relief of repression by adenovirus E1A protein [J].Cell,1991,67(2):377-388.
  • 3Chen QR,Yu LR,Tsang P,et al.Rystematic proteome analysis identifies transcription factor YY1 as a direct target of miR-34a[J].Proteome Res,2011,10(2):479-487.
  • 4Affarel B,Gay F,Shi Y,et al.Essential dosage-dependent functions of the transcription factor yin yang 1 in late embryonic development and cell cycle progression [J].Mol Cell Biol,2006,26(9):3565-3581.
  • 5Ishii H,Hulett MD,Li JM,et al.Yin Yang-1 inhibits tu-moreell growth and inhibits p21WAF1/Cip1 complex formation with cdk4 and cyclin D1 [J].Int J Oncol,2012,40(5):1575-1580.
  • 6Abbas T,Dutta A.p21 in cancer:intricate networks and multiple activities [ J ].Nat Rev Cancer,2009,9(6):400-414.
  • 7Roninson IB.Oncogenic functions of tumour suppressor p21(Waf1/Cip 1/Sdi 1):association with cell senescence and tumour-promoting activities of stromal fibroblasts[J].Cancer Lett,2002,179(1):1-14.
  • 8Sarbia M,Stahl M,zur Hausen A,et al.Expression of p21WAF1 predicts outcome of esophageal cancer pa-tients treated by surgery alone or by combined therapy modalities [ J ].Clin Cancer Res,1998,4(11):2615-2623.
  • 9Santiago FS,Ishii H,Shafi S,et al.Yin Yang-1 inhibits vascular smooth muscle cell growth and intimal thicken-ing by repressing p21WAF1/Cip1 transcription and p21WAF1/Cip1-Cdk4-cyclin D1 assembly[J].Circ Res,2007,101(2):146-155.
  • 10唐云,李雪丽,许雪珠.转录因子Yin—Yang1与肿瘤[J].国际肿瘤学杂志,2012,39(2):89-92. 被引量：1

二级参考文献26

  • 1Sakhinia E, Glennie C, Hoyland JA, et al. Clinical quantitation of diagnostic and predictive gene expression levels in follicular and dif- fuse large B-cell lymphoma by RT-PCR gene expression profiling. Blood, 2007, 109(9): 3922-3928.
  • 2Zaravinos A, Spandidos DA. Yin yang 1 expression in human tumors. Cell Cycle, 2010, 9(3): 512-522.
  • 3Caste|lano G, Torrisi E, Ligresti G, et al. The involvement of the transcription factor Yin Yang 1 in cancer development and progres- sion. Cell Cycle, 2009, 8(9) : 1367-1372.
  • 4Gr~nroos E, Terentive AA, Punga T, et al. YY1 inhibits the activa- tion of the p53 tumor suppressor in response to genotoxic stress. Proc Natl Acad Sei USA, 2004, 101 (33) : 12165-12170.
  • 5Gordon S, Akpyan G, Garban H, et al. Transcription factor YY1 : structure, function, and therapeutic implications in cancer biology. Oncogene, 2006, 25(8) : 1125-1142.
  • 6Wang CC, Tsai MF, Dai TH, et al. Synergistic activation of the tumor suppressor, HLJ1, by the transcription factors YY1 and acti- vator protein 1. Cancer Res, 2007, 67 (10) : 4816-4826.
  • 7Hongo F, Garban H, Huerta-Yepez S, et al. Inhibition of the tran-scription factor Yin Yang 1 activity by S-nitrosatio. Biochem Biophys Res Commun, 2005, 336(2) : 692-701.
  • 8Bonavida B, Baritaki S. Dual role of NO donors in the reversal of tumor ceil resistance and EMT:Downregulation of the NF-KB/Snail/ YY1/RKIP circuitry. Nitric Oxide, 2011, 24(1) : 1-7.
  • 9Baritaki S, Suzuki E, Umezawa K, et al. Inhibition of Yin Yang 1-dependent repressor activity of DR5 transcription and expression by the novel proteasome inhibitor NPI-O052 contributes to its TRAIL-en- hanced apoptosis in cancer ceils. J Immunol, 2008, 150(9) : 6199- 6210.
  • 10Baritaki S, Katsman A, Chatterjee D, et al. Regulation of tumor cell sensitivity to TRAIL-induced apoptosis by metastatic suppressor Raf kinase inhibitor protein via Yin Yang 1 inhibition and death receptor 5 up-regulation. J Immunol, 2007, 179(8): 5441-5453.

同被引文献27

  • 1逄旭光,葛棣,谢冬,侯英勇.VEGF、CD44v6和MMP-9在食管癌组织中的表达及临床意义[J].中国临床医学,2007,14(2):177-179. 被引量：5
  • 2Tao Y, Chai D, Ma L, et al. Identification of distinct gene ex- pression profiles between esophageal squamous cell carcinoma and adjacent normal epithelial tissues[J]. Tohoku J Exp Med, 2012, 226(4) ;301-311.
  • 3Luo J,Jiang X,Cao L, et al. Expression of YY1 correlates with progression and metastasis in esophageal squamous cell carcino- mas[J]. Onco Targets Ther, 2014, 26(7):1753-1759.
  • 4Zhang HQ, Wang RB, Yan H J, et al. Prognostic significance of CYFRA21-1, CEA and hemoglobin in patients with esophageal squamouscancer undergoing concurrent chemoradiotherapy[J]. Asian Pac J Cancer Prey, 2012, 13(1) :199-203.
  • 5Shen WD, Ji Y, Liu PF, et al. Correlation of E cadherin and CD44v6 expression with clinical pathology in esophageal carci- noma[J]. Mol Med Rep, 2012, 5(3) :817-821.
  • 6Allott EH, Lysaght J, Cathcart MC, et al. MMP9 expression in oesophageal adenocarcinoma is upregulated with visceral obesity and is associated with poor tumour differentiation[J] Mol Car- cinog, 2013, 52(2) :144-154.
  • 7Y. Lv,W. Wang,W.D. Jia,Q.K. Sun,M. Huang,H.C. Zhou,H.H. Xia,W.B. Liu,H. Chen,S.N. Sun,G.L. Xu.??High preoparative levels of serum periostin are associated with poor prognosis in patients with hepatocellular carcinoma after hepatectomy(J)European Journal of Surgical Oncology . 2013 (10)
  • 8Wang W,Sun Q K,He Y F, et al.Overexpression of periostin issignificantly correlated to the tumor angiogenesis and poor prognosis inpatients with esophageal squamous cell carcinoma. Int J Clin Exp Pathol . 2014
  • 9李斌,李劲松,袁宁.78例食管癌患者CK20 mRNA、CD44v6、NF-κB表达的临床意义[J].重庆医学,2011,40(20):1995-1996. 被引量：4
  • 10陈涛,骆金华,张憬.Ivor Lewis与左胸后外侧手术径路治疗食管癌的效果分析[J].南京医科大学学报（自然科学版）,2012,32(4):524-527. 被引量：10

引证文献3

二级引证文献12

南京医科大学学报（自然科学版）
2014年 第7期

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