血清miR-126在先天性心脏病患儿中的变化及临床意义

The changes of serum miR-126 in children with congenital heart disease and its clinical significance

目的：探讨血清miR-126的水平变化在先天性心脏病（CHD）患儿中的检测价值。方法：选取2013年1-6月本院心内科CHD患儿54例,其中左向右分流型先心病组（非发绀组）40例（心衰组20例,无心衰组20例）,右向左分流型先心病组（发绀组）14例,正常对照组20例。测定各组患儿血清中miR-126的水平,分析新生儿CHD血清miR-126与心力衰竭的相关性。结果：相比正常对照组,非发绀组和发绀组患儿血清miR-126表达水平显著降低（P〈0.05,P〈0.01）,且发绀组患儿血清miR-126表达水平低于非发绀组（P〈0.01）。在非发绀组患儿中,心衰组miR-126表达水平低于无心衰组（P〈0.001）。结论：CHD患儿血清miR-126显著降低,且病情越重降低越明显,提示miR-126可能参与了CHD的病变过程,可作为预测CHD风险的独立评价指标。

Objective：To determine miR-126 serum levels in children with congenital heart disease（CHD）. Methods：A total of 54children with CHD who received treatment in Department of Cardiology from January 2013 to June 2013 were enrolled. There were 40children with left-right shunt acyanotic CHD（the ACHD group）,including 15 children with heart failure（ACHDⅠ）and 25 children without heart failure（ACHDⅡ）. There were 14 children with right-left shunt cyanotic CHD（the CCHD group）and 20 healthy children（the control group）. The serum miR-126 expression level was measured to analyze the correlation between the serum miR-126 of neonates with CHD and heart failure. Results：Compared with normal controls,the expression levels of miR-126 in the ACHD group and the CCHD group were significantly down-regulated（P〈0.001）. The expression levels of miR-126 in the CCHD group were significantly lower than those in the ACHD group（P〈0.05 and P〈0.001,respectively）. MiR-126 expression levels were lower in the ACHDⅠ group those in the ACHDⅡ group（P〈0.001）. Conclusion：The expression levels of serum miR-126 in children with CHD were significantly decreased and correlated with the severity and degree of impaired cardiac function,indicating that miR-126 may be involved in the pathological process of CHD,and it may serve as an independent evaluation indicator for the diagnosis of CHD.